{"id":37949,"date":"2021-06-03T11:59:36","date_gmt":"2021-06-03T11:59:36","guid":{"rendered":"https:\/\/matis.is\/?p=37949"},"modified":"2021-06-03T11:59:38","modified_gmt":"2021-06-03T11:59:38","slug":"biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631","status":"publish","type":"post","link":"https:\/\/matis.is\/en\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/","title":{"rendered":"Biochemical characterization and validation of a catalytic site of a highly thermostable Ts2631 endolysin from the Thermus scotoductus phage vB_Tsc2631"},"content":{"rendered":"<p>Phage vB_Tsc2631 infects the extremophilic bacterium\u00a0<em>Thermus scotoductus<\/em>\u00a0MAT2631 and uses the Ts2631 endolysin for the release of its progeny. The Ts2631 endolysin is the first endolysin from thermophilic bacteriophage with an experimentally validated catalytic site.\u00a0<em>In silico<\/em>\u00a0analysis and computational modeling of the Ts2631 endolysin structure revealed a conserved Zn<sup>2+<\/sup>\u00a0binding site (His<sup>30<\/sup>, Tyr<sup>58<\/sup>, His<sup>131<\/sup>\u00a0and Cys<sup>139<\/sup>) similar to Zn<sup>2+<\/sup>\u00a0binding site of eukaryotic peptidoglycan recognition proteins (PGRPs). We have shown that the Ts2631 endolysin lytic activity is dependent on divalent metal ions (Zn<sup>2+<\/sup>\u00a0and Ca<sup>2+<\/sup>). The Ts2631 endolysin substitution variants H30N, Y58F, H131N and C139S dramatically lost their antimicrobial activity, providing evidence for the role of the aforementioned residues in the lytic activity of the enzyme. The enzyme has proven to be not only thermoresistant, retaining 64.8% of its initial activity after 2 h at 95 \u00b0 C, but also highly thermodynamically stable (T<sub>m<\/sub>\u00a0= 99.82 \u00b0 C, \u0394H<sub>cal<\/sub>\u00a0= 4.58 \u00d7 10<sup>4<\/sup>\u00a0cal mol<sup>-1<\/sup>). Substitutions of histidine residues (H30N and H131N) and a cysteine residue (C139S) resulted in variants aggregating at temperatures \u226575 \u00b0 C, indicating a significant role of these residues in enzyme thermostability. The substrate spectrum of the Ts2631 endolysin included extremophiles of the genus\u00a0<em>Thermus<\/em>\u00a0but also Gram-negative mesophiles, such as\u00a0<em>Escherichia coli<\/em>,\u00a0<em>Salmonella panama<\/em>,\u00a0<em>Pseudomonas fluorescens<\/em>\u00a0and\u00a0<em>Serratia marcescens<\/em>. The broad substrate spectrum and high thermostability of this endolysin makes it a good candidate for use as an antimicrobial agent to combat Gram-negative pathogens.<\/p>\n\n\n\n<p><strong><a href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0137374\">Link to article<\/a><\/strong><\/p>","protected":false},"excerpt":{"rendered":"<p>Phage vB_Tsc2631 infects the extremophilic bacterium\u00a0Thermus scotoductus\u00a0MAT2631 and uses the Ts2631 endolysin for the release of its progeny. The Ts2631 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"template-greinar.php","format":"standard","meta":{"_acf_changed":false,"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[2011],"tags":[],"class_list":["post-37949","post","type-post","status-publish","format-standard","hentry","category-greinar"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Biochemical characterization and validation of a catalytic site of a highly thermostable Ts2631 endolysin from the Thermus scotoductus phage vB_Tsc2631 - Mat\u00eds<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/matis.is\/en\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Biochemical characterization and validation of a catalytic site of a highly thermostable Ts2631 endolysin from the Thermus scotoductus phage vB_Tsc2631 - Mat\u00eds\" \/>\n<meta property=\"og:description\" content=\"Phage vB_Tsc2631 infects the extremophilic bacterium\u00a0Thermus scotoductus\u00a0MAT2631 and uses the Ts2631 endolysin for the release of its progeny. The Ts2631 [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/matis.is\/en\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/\" \/>\n<meta property=\"og:site_name\" content=\"Mat\u00eds\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/matisiceland\" \/>\n<meta property=\"article:published_time\" content=\"2021-06-03T11:59:36+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2021-06-03T11:59:38+00:00\" \/>\n<meta name=\"author\" content=\"admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@matisiceland\" \/>\n<meta name=\"twitter:site\" content=\"@matisiceland\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"admin\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/\"},\"author\":{\"name\":\"admin\",\"@id\":\"https:\/\/matis.is\/#\/schema\/person\/33b92c850c915c93e67f291e3381a49a\"},\"headline\":\"Biochemical characterization and validation of a catalytic site of a highly thermostable Ts2631 endolysin from the Thermus scotoductus phage vB_Tsc2631\",\"datePublished\":\"2021-06-03T11:59:36+00:00\",\"dateModified\":\"2021-06-03T11:59:38+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/\"},\"wordCount\":260,\"publisher\":{\"@id\":\"https:\/\/matis.is\/#organization\"},\"articleSection\":[\"Greinar\"],\"inLanguage\":\"en-GB\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/\",\"url\":\"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/\",\"name\":\"Biochemical characterization and validation of a catalytic site of a highly thermostable Ts2631 endolysin from the Thermus scotoductus phage vB_Tsc2631 - Mat\u00eds\",\"isPartOf\":{\"@id\":\"https:\/\/matis.is\/#website\"},\"datePublished\":\"2021-06-03T11:59:36+00:00\",\"dateModified\":\"2021-06-03T11:59:38+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/matis.is\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Biochemical characterization and validation of a catalytic site of a highly thermostable Ts2631 endolysin from the Thermus scotoductus phage vB_Tsc2631\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/matis.is\/#website\",\"url\":\"https:\/\/matis.is\/\",\"name\":\"Mat\u00eds\",\"description\":\"Ver\u00f0m\u00e6task\u00f6pun, matv\u00e6la\u00f6ryggi og l\u00fd\u00f0heilsa\",\"publisher\":{\"@id\":\"https:\/\/matis.is\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/matis.is\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-GB\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/matis.is\/#organization\",\"name\":\"Mat\u00eds ohf.\",\"url\":\"https:\/\/matis.is\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\/\/matis.is\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png\",\"contentUrl\":\"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png\",\"width\":1507,\"height\":1507,\"caption\":\"Mat\u00eds ohf.\"},\"image\":{\"@id\":\"https:\/\/matis.is\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/www.facebook.com\/matisiceland\",\"https:\/\/x.com\/matisiceland\",\"https:\/\/www.instagram.com\/matisiceland\/\",\"https:\/\/www.linkedin.com\/matisiceland\/\",\"https:\/\/www.youtube.com\/channel\/UCY-frt3uTqgVZW-DLjoo5bA\"]},{\"@type\":\"Person\",\"@id\":\"https:\/\/matis.is\/#\/schema\/person\/33b92c850c915c93e67f291e3381a49a\",\"name\":\"admin\",\"sameAs\":[\"https:\/\/matis.mareframe.eu\"],\"url\":\"https:\/\/matis.is\/en\/author\/thormodurmatis-is\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Biochemical characterization and validation of a catalytic site of a highly thermostable Ts2631 endolysin from the Thermus scotoductus phage vB_Tsc2631 - Mat\u00eds","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/matis.is\/en\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/","og_locale":"en_GB","og_type":"article","og_title":"Biochemical characterization and validation of a catalytic site of a highly thermostable Ts2631 endolysin from the Thermus scotoductus phage vB_Tsc2631 - Mat\u00eds","og_description":"Phage vB_Tsc2631 infects the extremophilic bacterium\u00a0Thermus scotoductus\u00a0MAT2631 and uses the Ts2631 endolysin for the release of its progeny. The Ts2631 [&hellip;]","og_url":"https:\/\/matis.is\/en\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/","og_site_name":"Mat\u00eds","article_publisher":"https:\/\/www.facebook.com\/matisiceland","article_published_time":"2021-06-03T11:59:36+00:00","article_modified_time":"2021-06-03T11:59:38+00:00","author":"admin","twitter_card":"summary_large_image","twitter_creator":"@matisiceland","twitter_site":"@matisiceland","twitter_misc":{"Written by":"admin","Estimated reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/#article","isPartOf":{"@id":"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/"},"author":{"name":"admin","@id":"https:\/\/matis.is\/#\/schema\/person\/33b92c850c915c93e67f291e3381a49a"},"headline":"Biochemical characterization and validation of a catalytic site of a highly thermostable Ts2631 endolysin from the Thermus scotoductus phage vB_Tsc2631","datePublished":"2021-06-03T11:59:36+00:00","dateModified":"2021-06-03T11:59:38+00:00","mainEntityOfPage":{"@id":"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/"},"wordCount":260,"publisher":{"@id":"https:\/\/matis.is\/#organization"},"articleSection":["Greinar"],"inLanguage":"en-GB"},{"@type":"WebPage","@id":"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/","url":"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/","name":"Biochemical characterization and validation of a catalytic site of a highly thermostable Ts2631 endolysin from the Thermus scotoductus phage vB_Tsc2631 - Mat\u00eds","isPartOf":{"@id":"https:\/\/matis.is\/#website"},"datePublished":"2021-06-03T11:59:36+00:00","dateModified":"2021-06-03T11:59:38+00:00","breadcrumb":{"@id":"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/#breadcrumb"},"inLanguage":"en-GB","potentialAction":[{"@type":"ReadAction","target":["https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/matis.is\/greinar\/biochemical-characterization-and-validation-of-a-catalytic-site-of-a-highly-thermostable-ts2631-endolysin-from-the-thermus-scotoductus-phage-vb_tsc2631\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/matis.is\/"},{"@type":"ListItem","position":2,"name":"Biochemical characterization and validation of a catalytic site of a highly thermostable Ts2631 endolysin from the Thermus scotoductus phage vB_Tsc2631"}]},{"@type":"WebSite","@id":"https:\/\/matis.is\/#website","url":"https:\/\/matis.is\/","name":"Mat\u00eds","description":"Value creation, food security and public health","publisher":{"@id":"https:\/\/matis.is\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/matis.is\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-GB"},{"@type":"Organization","@id":"https:\/\/matis.is\/#organization","name":"Mat\u00eds ohf.","url":"https:\/\/matis.is\/","logo":{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/matis.is\/#\/schema\/logo\/image\/","url":"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png","contentUrl":"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png","width":1507,"height":1507,"caption":"Mat\u00eds ohf."},"image":{"@id":"https:\/\/matis.is\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/matisiceland","https:\/\/x.com\/matisiceland","https:\/\/www.instagram.com\/matisiceland\/","https:\/\/www.linkedin.com\/matisiceland\/","https:\/\/www.youtube.com\/channel\/UCY-frt3uTqgVZW-DLjoo5bA"]},{"@type":"Person","@id":"https:\/\/matis.is\/#\/schema\/person\/33b92c850c915c93e67f291e3381a49a","name":"admin","sameAs":["https:\/\/matis.mareframe.eu"],"url":"https:\/\/matis.is\/en\/author\/thormodurmatis-is\/"}]}},"spectra_custom_meta":{"_edit_lock":["1622721594:5"],"_wp_page_template":["template-greinar.php"],"_edit_last":["5"],"_yoast_wpseo_content_score":["30"],"_yoast_wpseo_estimated-reading-time-minutes":["2"],"authors":["Plotka, M, Kaczorowska A-K, Morzywolek A, Makowska J, Kozlowski LP, Thorisdottir A,\u00a0Sk\u00edrnisdottir S,\u00a0Hj\u00f6rleifsdottir S,\u00a0Fridjonsson OH,\u00a0Hreggvidsson GO, Kristjansson JK, Dabrowski S, Bujnicki JM, Kaczorowski T."],"_authors":["field_60a287218a0ab"],"publication":["PlosOne"],"_publication":["field_60a287738a0ac"],"edition":["10(9)"],"_edition":["field_60a287918a0ad"],"page":["e0137374"],"_page":["field_60a2879f8a0ae"],"year":["2015"],"_year":["field_60a287a58a0af"],"link":[""],"_link":["field_60a287aa8a0b0"],"orcidid":[""],"_orcidid":["field_60a287c28a0b1"],"matis_tengilidur":["a:3:{i:0;s:5:\"28287\";i:1;s:5:\"28263\";i:2;s:5:\"28169\";}"],"_matis_tengilidur":["field_60a65b61f4532"],"tengilidur":[""],"_tengilidur":["field_5ef905e0538b2"],"_yoast_wpseo_primary_category":["2011"],"wpmm_postgrid_views":["574"],"_uag_css_file_name":["uag-css-37949.css"],"_uag_page_assets":["a:9:{s:3:\"css\";s:263:\".uag-blocks-common-selector{z-index:var(--z-index-desktop) !important}@media (max-width: 976px){.uag-blocks-common-selector{z-index:var(--z-index-tablet) !important}}@media (max-width: 767px){.uag-blocks-common-selector{z-index:var(--z-index-mobile) !important}}\n\";s:2:\"js\";s:0:\"\";s:18:\"current_block_list\";a:6:{i:0;s:14:\"core\/paragraph\";i:1;s:12:\"core\/buttons\";i:2;s:11:\"core\/button\";i:3;s:12:\"core\/heading\";i:4;s:12:\"core\/columns\";i:5;s:11:\"core\/column\";}s:8:\"uag_flag\";b:0;s:11:\"uag_version\";s:10:\"1775648310\";s:6:\"gfonts\";a:0:{}s:10:\"gfonts_url\";s:0:\"\";s:12:\"gfonts_files\";a:0:{}s:14:\"uag_faq_layout\";b:0;}"]},"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false},"uagb_author_info":{"display_name":"admin","author_link":"https:\/\/matis.is\/en\/author\/thormodurmatis-is\/"},"uagb_comment_info":0,"uagb_excerpt":"Phage vB_Tsc2631 infects the extremophilic bacterium\u00a0Thermus scotoductus\u00a0MAT2631 and uses the Ts2631 endolysin for the release of its progeny. The Ts2631 [&hellip;]","_links":{"self":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts\/37949","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/comments?post=37949"}],"version-history":[{"count":2,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts\/37949\/revisions"}],"predecessor-version":[{"id":37951,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts\/37949\/revisions\/37951"}],"wp:attachment":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/media?parent=37949"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/categories?post=37949"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/tags?post=37949"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}