{"id":37970,"date":"2021-06-03T12:49:29","date_gmt":"2021-06-03T12:49:29","guid":{"rendered":"https:\/\/matis.is\/?p=37970"},"modified":"2021-06-03T12:49:31","modified_gmt":"2021-06-03T12:49:31","slug":"preventive-effect-of-nile-tilapia-hydrolysate-against-oxidative-damage-of-hepg2-cells-and-dna-mediated-y-h202-and-aaph","status":"publish","type":"post","link":"https:\/\/matis.is\/en\/greinar\/preventive-effect-of-nile-tilapia-hydrolysate-against-oxidative-damage-of-hepg2-cells-and-dna-mediated-y-h202-and-aaph\/","title":{"rendered":"Preventive effect of Nile tilapia hydrolyzate against oxidative damage of HepG2 cells and DNA mediated y H202 and AAPH"},"content":{"rendered":"<p class=\"wp-block-paragraph\">Antioxidant activities of protein hydrolyzate prepared from Nile tilapia protein isolate using Alcalase (HA), Alcalase followed by papain (HAPa) and their Sephadex G-25 fractions (FHA and FHAPa) were investigated in both chemical and cellular based models. Amongst all samples, FHAPa showed the highest chemical antioxidant activities, however it had no metal chelation activity. Cellular antioxidant ability of HA, HAPa and their fractions against H<sub>2<\/sub>O<sub>2<\/sub>\u00a0and AAPH induced oxidative damage of HepG2 cell and DNA were tested. When cells were pretreated with all hydrolysates or fractions at different concentrations (0.5\u20132 mg \/ mL) in the absence and presence of 50 \u03bcM Trolox, cell viability was in the range of 91.10\u2013111.40 %. However, no difference in cell viability was observed among samples having various concentrations (<em>P<\/em>&gt; 0.05). Cell reactive oxygen species (ROS) generation as mediated by H<sub>2<\/sub>O<sub>2<\/sub>\u00a0and AAPH decreased with treatment of hydrolysates or their fractions, especially in combination with 50 \u03bcM Trolox. FHAPa effectively inhibited H<sub>2<\/sub>O<sub>2<\/sub>\u00a0and peroxyl radical induced DNA scission in a dose dependent manner. Therefore, Nile tilapia protein hydrolysates could serve as a functional food ingredient.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><a href=\"https:\/\/link.springer.com\/article\/10.1007%2Fs13197-014-1672-4\">Link to article<\/a><\/strong><\/p>","protected":false},"excerpt":{"rendered":"<p>Antioxidant activities of protein hydrolysate prepared from Nile tilapia protein isolate using Alcalase (HA), Alcalase followed by papain (HAPa) and [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"template-greinar.php","format":"standard","meta":{"_acf_changed":false,"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[2011],"tags":[],"class_list":["post-37970","post","type-post","status-publish","format-standard","hentry","category-greinar"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.7 - 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