{"id":38379,"date":"2011-06-07T13:20:00","date_gmt":"2011-06-07T13:20:00","guid":{"rendered":"https:\/\/matis.is\/?p=38379"},"modified":"2023-02-03T12:39:32","modified_gmt":"2023-02-03T12:39:32","slug":"comparison-of-campylobacter-jejuni-isolates-from-human-food-veterinary-and-environmental-sources-in-iceland-using-pfge-mlst-and-fla-svr-sequencing","status":"publish","type":"post","link":"https:\/\/matis.is\/en\/greinar\/comparison-of-campylobacter-jejuni-isolates-from-human-food-veterinary-and-environmental-sources-in-iceland-using-pfge-mlst-and-fla-svr-sequencing\/","title":{"rendered":"Comparison of Campylobacter jejuni isolates from human, food, veterinary and environmental sources in Iceland using PFGE, MLST and fla-SVR sequencing"},"content":{"rendered":"<p><strong>Aims:&nbsp;<\/strong><em>Campylobacter jejuni<\/em>&nbsp;isolates from various sources in Iceland were genotyped with the aim of assessing the genetic diversity, population structure, source distribution and campylobacter transmission routes to humans.<\/p>\n\n\n\n<p><strong>Methods and Results:&nbsp;<\/strong>A collection of 584&nbsp;<em>Campylobacter<\/em>&nbsp;isolates were collected from clinical cases, food, animals and environment in Iceland in 1999\u20132002, during a period of national&nbsp;<em>Campylobacter<\/em>&nbsp;epidemic in Iceland. All isolates were characterized by pulse field gel electrophoresis (PFGE), and selected subset of 52 isolates representing the diversity of the identified PFGE types was further genotyped using multilocus sequence typing (MLST) and&nbsp;<em>fla<\/em>-SVR sequencing to gain better insight into the population structure.<\/p>\n\n\n\n<p><strong>Conclusions:&nbsp;<\/strong>The results show a substantial diversity within the Icelandic&nbsp;<em>Campylobacter<\/em>&nbsp;population. Majority of the human&nbsp;<em>Campylobacter<\/em>&nbsp;infections originated from domestic chicken and cattle isolates. MLST showed the isolates to be distributed among previously reported and common sequence type complexes in the MLST database.<\/p>\n\n\n\n<p><strong>Significance and Impact of the Study:&nbsp;<\/strong>The genotyping of&nbsp;<em>Campylobacter<\/em>&nbsp;from various sources has not previously been reported from Iceland, and the results of the study gave a valuable insight into the population structure of&nbsp;<em>Camp. jejuni<\/em>&nbsp;in Iceland, source distribution and transmission routes to humans. The geographical isolation of Iceland in the north Atlantic provides new information on&nbsp;<em>Campylobacter<\/em>&nbsp;population dynamics on a global scale.<\/p>\n\n\n\n<p><strong><a href=\"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/full\/10.1111\/j.1365-2672.2011.05100.x\">Link to article<\/a><\/strong><\/p>","protected":false},"excerpt":{"rendered":"<p>Aims:&nbsp;Campylobacter jejuni&nbsp;isolates from various sources in Iceland were genotyped with the aim of assessing the genetic diversity, population structure, source [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"template-greinar.php","format":"standard","meta":{"_acf_changed":false,"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[2011],"tags":[],"class_list":["post-38379","post","type-post","status-publish","format-standard","hentry","category-greinar"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - 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