{"id":38725,"date":"2021-06-08T15:16:32","date_gmt":"2021-06-08T15:16:32","guid":{"rendered":"https:\/\/matis.is\/?p=38725"},"modified":"2021-06-08T15:16:32","modified_gmt":"2021-06-08T15:16:32","slug":"characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose","status":"publish","type":"post","link":"https:\/\/matis.is\/en\/greinar\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\/","title":{"rendered":"Characterization of an extracellular vibriolysin of the fish pathogen Moritella viscose"},"content":{"rendered":"<p><em>Moritella viscosa<\/em>\u00a0causes winter ulcer disease in salmonids. The aim of the present work was to isolate and partially characterize an extracellular peptidase from\u00a0<em>M. viscosa<\/em>, and to study its role in virulence. The peptidase, termed MvP1, was a 38-kDa metallopeptidase produced in late exponential growth. The optimum temperature for MvP1 was 40 \u00b0 C, but the enzyme was active over a wide range of temperatures. MvP1 was non-lethal to salmon at concentrations up to 0.22 \u03bcg \/ g fish, but extracellular products were lethal to salmon. MvP1 degraded casein, gelatin and collagen from lumpfish skin. It caused considerable tissue necrosis and hemorrhages at the site of injection, and affected cell \u2013 cell adhesions in EPC and BF-2 cell lines, but was not highly cytotoxic. The peptidase partially degraded fish IgM heavy chain but was non-hemolytic. The\u00a0<em>mvp1<\/em>\u00a0gene was sequenced and encoded a 734-aa polypeptide containing a signal sequence, an N-terminal propeptide, a mature peptidase domain and a C-terminal propeptide. The MvP1 propeptide undergoes both N-terminal and C-terminal processing and different C-terminal processing results in the formation of several active isoforms of the mature peptidase. The catalytic domain showed highest sequence similarity with several vibriolysins (EC 3.4.24.25) originating from\u00a0<em>Pseudoalteromonas<\/em>\u00a0strains, showing up to 80% aa identity. The results indicate that MvP1 is a previously unknown vibriolysin that might affect\u00a0<em>M. viscosa<\/em>\u00a0virulence by aiding in the invasion and dissemination of the bacterium in its host, by causing tissue destruction.<\/p>\n\n\n\n<p><strong><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0378113508005518?via%3Dihub\">Link to article<\/a><\/strong><\/p>","protected":false},"excerpt":{"rendered":"<p>Moritella viscosa\u00a0causes winter ulcer disease in salmonids. The aim of the present work was to isolate and partially characterise an [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"template-greinar.php","format":"standard","meta":{"_acf_changed":false,"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[2011],"tags":[],"class_list":["post-38725","post","type-post","status-publish","format-standard","hentry","category-greinar"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Characterization of an extracellular vibriolysin of the fish pathogen\u00a0Moritella viscose - Mat\u00eds<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/matis.is\/en\/greinar\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Characterization of an extracellular vibriolysin of the fish pathogen\u00a0Moritella viscose - Mat\u00eds\" \/>\n<meta property=\"og:description\" content=\"Moritella viscosa\u00a0causes winter ulcer disease in salmonids. The aim of the present work was to isolate and partially characterise an [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/matis.is\/en\/greinar\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\/\" \/>\n<meta property=\"og:site_name\" content=\"Mat\u00eds\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/matisiceland\" \/>\n<meta property=\"article:published_time\" content=\"2021-06-08T15:16:32+00:00\" \/>\n<meta name=\"author\" content=\"admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@matisiceland\" \/>\n<meta name=\"twitter:site\" content=\"@matisiceland\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"admin\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/matis.is\\\/greinar\\\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/matis.is\\\/greinar\\\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\\\/\"},\"author\":{\"name\":\"admin\",\"@id\":\"https:\\\/\\\/matis.is\\\/#\\\/schema\\\/person\\\/33b92c850c915c93e67f291e3381a49a\"},\"headline\":\"Characterization of an extracellular vibriolysin of the fish pathogen\u00a0Moritella viscose\",\"datePublished\":\"2021-06-08T15:16:32+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/matis.is\\\/greinar\\\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\\\/\"},\"wordCount\":248,\"publisher\":{\"@id\":\"https:\\\/\\\/matis.is\\\/#organization\"},\"articleSection\":[\"Greinar\"],\"inLanguage\":\"en-GB\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/matis.is\\\/greinar\\\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\\\/\",\"url\":\"https:\\\/\\\/matis.is\\\/greinar\\\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\\\/\",\"name\":\"Characterization of an extracellular vibriolysin of the fish pathogen\u00a0Moritella viscose - Mat\u00eds\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/matis.is\\\/#website\"},\"datePublished\":\"2021-06-08T15:16:32+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/matis.is\\\/greinar\\\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\\\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/matis.is\\\/greinar\\\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/matis.is\\\/greinar\\\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/matis.is\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Characterization of an extracellular vibriolysin of the fish pathogen\u00a0Moritella viscose\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/matis.is\\\/#website\",\"url\":\"https:\\\/\\\/matis.is\\\/\",\"name\":\"Mat\u00eds\",\"description\":\"Ver\u00f0m\u00e6task\u00f6pun, matv\u00e6la\u00f6ryggi og l\u00fd\u00f0heilsa\",\"publisher\":{\"@id\":\"https:\\\/\\\/matis.is\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/matis.is\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-GB\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/matis.is\\\/#organization\",\"name\":\"Mat\u00eds ohf.\",\"url\":\"https:\\\/\\\/matis.is\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\\\/\\\/matis.is\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/matis.is\\\/wp-content\\\/uploads\\\/2021\\\/02\\\/matis_logo_blatthvitt.png\",\"contentUrl\":\"https:\\\/\\\/matis.is\\\/wp-content\\\/uploads\\\/2021\\\/02\\\/matis_logo_blatthvitt.png\",\"width\":1507,\"height\":1507,\"caption\":\"Mat\u00eds ohf.\"},\"image\":{\"@id\":\"https:\\\/\\\/matis.is\\\/#\\\/schema\\\/logo\\\/image\\\/\"},\"sameAs\":[\"https:\\\/\\\/www.facebook.com\\\/matisiceland\",\"https:\\\/\\\/x.com\\\/matisiceland\",\"https:\\\/\\\/www.instagram.com\\\/matisiceland\\\/\",\"https:\\\/\\\/www.linkedin.com\\\/matisiceland\\\/\",\"https:\\\/\\\/www.youtube.com\\\/channel\\\/UCY-frt3uTqgVZW-DLjoo5bA\"]},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/matis.is\\\/#\\\/schema\\\/person\\\/33b92c850c915c93e67f291e3381a49a\",\"name\":\"admin\",\"sameAs\":[\"https:\\\/\\\/matis.mareframe.eu\"],\"url\":\"https:\\\/\\\/matis.is\\\/en\\\/author\\\/thormodurmatis-is\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Characterization of an extracellular vibriolysin of the fish pathogen Moritella viscose - Mat\u00eds","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/matis.is\/en\/greinar\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\/","og_locale":"en_GB","og_type":"article","og_title":"Characterization of an extracellular vibriolysin of the fish pathogen\u00a0Moritella viscose - Mat\u00eds","og_description":"Moritella viscosa\u00a0causes winter ulcer disease in salmonids. The aim of the present work was to isolate and partially characterise an [&hellip;]","og_url":"https:\/\/matis.is\/en\/greinar\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\/","og_site_name":"Mat\u00eds","article_publisher":"https:\/\/www.facebook.com\/matisiceland","article_published_time":"2021-06-08T15:16:32+00:00","author":"admin","twitter_card":"summary_large_image","twitter_creator":"@matisiceland","twitter_site":"@matisiceland","twitter_misc":{"Written by":"admin","Estimated reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/matis.is\/greinar\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\/#article","isPartOf":{"@id":"https:\/\/matis.is\/greinar\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\/"},"author":{"name":"admin","@id":"https:\/\/matis.is\/#\/schema\/person\/33b92c850c915c93e67f291e3381a49a"},"headline":"Characterization of an extracellular vibriolysin of the fish pathogen\u00a0Moritella viscose","datePublished":"2021-06-08T15:16:32+00:00","mainEntityOfPage":{"@id":"https:\/\/matis.is\/greinar\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\/"},"wordCount":248,"publisher":{"@id":"https:\/\/matis.is\/#organization"},"articleSection":["Greinar"],"inLanguage":"en-GB"},{"@type":"WebPage","@id":"https:\/\/matis.is\/greinar\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\/","url":"https:\/\/matis.is\/greinar\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\/","name":"Characterization of an extracellular vibriolysin of the fish pathogen Moritella viscose - Mat\u00eds","isPartOf":{"@id":"https:\/\/matis.is\/#website"},"datePublished":"2021-06-08T15:16:32+00:00","breadcrumb":{"@id":"https:\/\/matis.is\/greinar\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\/#breadcrumb"},"inLanguage":"en-GB","potentialAction":[{"@type":"ReadAction","target":["https:\/\/matis.is\/greinar\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/matis.is\/greinar\/characterization-of-an-extracellular-vibriolysin-of-the-fish-pathogen-moritella-viscose\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/matis.is\/"},{"@type":"ListItem","position":2,"name":"Characterization of an extracellular vibriolysin of the fish pathogen\u00a0Moritella viscose"}]},{"@type":"WebSite","@id":"https:\/\/matis.is\/#website","url":"https:\/\/matis.is\/","name":"Mat\u00eds","description":"Value creation, food security and public health","publisher":{"@id":"https:\/\/matis.is\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/matis.is\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-GB"},{"@type":"Organization","@id":"https:\/\/matis.is\/#organization","name":"Mat\u00eds ohf.","url":"https:\/\/matis.is\/","logo":{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/matis.is\/#\/schema\/logo\/image\/","url":"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png","contentUrl":"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png","width":1507,"height":1507,"caption":"Mat\u00eds ohf."},"image":{"@id":"https:\/\/matis.is\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/matisiceland","https:\/\/x.com\/matisiceland","https:\/\/www.instagram.com\/matisiceland\/","https:\/\/www.linkedin.com\/matisiceland\/","https:\/\/www.youtube.com\/channel\/UCY-frt3uTqgVZW-DLjoo5bA"]},{"@type":"Person","@id":"https:\/\/matis.is\/#\/schema\/person\/33b92c850c915c93e67f291e3381a49a","name":"admin","sameAs":["https:\/\/matis.mareframe.eu"],"url":"https:\/\/matis.is\/en\/author\/thormodurmatis-is\/"}]}},"spectra_custom_meta":{"_edit_lock":["1623165406:5"],"_wp_page_template":["template-greinar.php"],"_edit_last":["5"],"_yoast_wpseo_content_score":["30"],"_yoast_wpseo_estimated-reading-time-minutes":["2"],"authors":["Bryndis Bjornsdottir,\u00a0\u00d3lafur H. Fri\u00f0j\u00f3nsson,\u00a0Steinunn Magn\u00fasd\u00f3ttir, Valger\u00f0ur Andr\u00e9sd\u00f3ttir,\u00a0Gu\u00f0mundur \u00d3. Hreggvi\u00f0sson,\u00a0and Bjarnhei\u00f0ur K. Gu\u00f0mundsd\u00f3ttir"],"_authors":["field_60a287218a0ab"],"publication":["Veterinary Microbiology"],"_publication":["field_60a287738a0ac"],"edition":["136(3-4)"],"_edition":["field_60a287918a0ad"],"page":["326-334"],"_page":["field_60a2879f8a0ae"],"year":["2009"],"_year":["field_60a287a58a0af"],"link":[""],"_link":["field_60a287aa8a0b0"],"orcidid":[""],"_orcidid":["field_60a287c28a0b1"],"matis_tengilidur":["a:3:{i:0;s:5:\"28147\";i:1;s:5:\"28263\";i:2;s:5:\"28169\";}"],"_matis_tengilidur":["field_60a65b61f4532"],"tengilidur":[""],"_tengilidur":["field_5ef905e0538b2"],"_yoast_wpseo_primary_category":["2011"],"wpmm_postgrid_views":["593"],"_uag_css_file_name":["uag-css-38725.css"],"_uag_page_assets":["a:9:{s:3:\"css\";s:263:\".uag-blocks-common-selector{z-index:var(--z-index-desktop) !important}@media (max-width: 976px){.uag-blocks-common-selector{z-index:var(--z-index-tablet) !important}}@media (max-width: 767px){.uag-blocks-common-selector{z-index:var(--z-index-mobile) !important}}\n\";s:2:\"js\";s:0:\"\";s:18:\"current_block_list\";a:6:{i:0;s:14:\"core\/paragraph\";i:1;s:12:\"core\/buttons\";i:2;s:11:\"core\/button\";i:3;s:12:\"core\/heading\";i:4;s:12:\"core\/columns\";i:5;s:11:\"core\/column\";}s:8:\"uag_flag\";b:0;s:11:\"uag_version\";s:10:\"1775648310\";s:6:\"gfonts\";a:0:{}s:10:\"gfonts_url\";s:0:\"\";s:12:\"gfonts_files\";a:0:{}s:14:\"uag_faq_layout\";b:0;}"]},"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false},"uagb_author_info":{"display_name":"admin","author_link":"https:\/\/matis.is\/en\/author\/thormodurmatis-is\/"},"uagb_comment_info":0,"uagb_excerpt":"Moritella viscosa\u00a0causes winter ulcer disease in salmonids. The aim of the present work was to isolate and partially characterise an [&hellip;]","_links":{"self":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts\/38725","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/comments?post=38725"}],"version-history":[{"count":2,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts\/38725\/revisions"}],"predecessor-version":[{"id":38727,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts\/38725\/revisions\/38727"}],"wp:attachment":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/media?parent=38725"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/categories?post=38725"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/tags?post=38725"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}