{"id":42706,"date":"2010-09-21T09:27:00","date_gmt":"2010-09-21T09:27:00","guid":{"rendered":"https:\/\/matis.is\/?p=42706"},"modified":"2021-08-23T09:29:07","modified_gmt":"2021-08-23T09:29:07","slug":"doktorsvorn-i-liffraedi-erfdabreytingar-a-bakteriunni-rhodothermus-marinus","status":"publish","type":"post","link":"https:\/\/matis.is\/en\/frettir\/doktorsvorn-i-liffraedi-erfdabreytingar-a-bakteriunni-rhodothermus-marinus\/","title":{"rendered":"Doctoral dissertation in biology: Genetic modification of the bacterium Rhodothermus marinus"},"content":{"rendered":"

On Monday 27 September, an employee of Mat\u00eds will, Sn\u00e6d\u00eds Huld Bj\u00f6rnsd\u00f3ttir,<\/strong> defend his doctoral dissertation \u201cGenetic changes in the bacterium Rhodothermus marinus<\/em>\u201c(E. Genetic engineering of Rhodothermus marinus<\/em>).<\/h4>\n\n\n\n

Supervisors were Gu\u00f0mundur Eggertsson<\/strong> Professor Emeritus and \u00d3lafur S. Andr\u00e9sson<\/strong> professor. In addition, Dr. Jakob K. Kristj\u00e1nsson<\/strong> CEO of Arkea, Sigr\u00ed\u00f0ur H. \u00deorbjarnard\u00f3ttir<\/strong> expert at the Institute of Biology and Dr. \u00d3lafur H. Fri\u00f0j\u00f3nsson<\/strong>, project manager at Mat\u00eds. <\/p>\n\n\n\n

Opponents are Daniel Prieur<\/strong>, Professor at the Universit\u00e9 de Bretagne Occidentale in Brest, France and Dr. Valger\u00f0ur Andr\u00e9sd\u00f3ttir<\/strong>, scientist at the University of Iceland Laboratory of Pathology at Keldur.<\/p>\n\n\n\n

Sn\u00e6d\u00eds was born in 1973. She has worked at the Institute of Biology, at Prokaria and as a specialist at Mat\u00eds 'Department of Biotechnology and Biochemistry since 2007. Sn\u00e6d\u00eds' research focused on the development of methods to genetically modify the thermophilic bacterium. Rhodothermus marinus<\/em>. Sn\u00e6d\u00eds 'husband is \u00c6gir \u00de\u00f3r \u00de\u00f3rsson, a horticultural consultant at the Icelandic Farmers' Association. <\/p>\n\n\n\n

Vice President of the Faculty of Life and Environmental Sciences, Karl Benediktsson<\/strong> professor, presides over the ceremony requested The main hall of the main building<\/strong> and starts at 13:00<\/strong>.<\/p>\n\n\n\n

Abstract<\/strong>
Rhodothermus marinus<\/em> is an aerobic, thermophilic bacterium that was first isolated from underwater hot springs at \u00cdsafjar\u00f0ardj\u00fap. The species is interesting, partly because of its position in the bacterial classification system and because of its adaptation to its natural environment. In addition produces R. marinus<\/em> a large collection of heat-resistant enzymes that can be used in industry. These include enzymes that break down polysaccharides and biomass. Here's another one Rhodothermus<\/em> type described. It was isolated from a depth of 2634 m in the Pacific Ocean and was named R. profundi. <\/em><\/p>\n\n\n\n

The main objective of this project was the development of methods for genetic modification R. marinus<\/em>. A method was invented to transfer foreign genes into the bacterium. Two options were used, trpB<\/em> and purA,<\/em> but they list for enzymes involved in the synthesis of tryptophan and adenine.  R. marinus<\/em> a strain that did not appear to have curvature activity was selected for genetic uptake. Both trpB<\/em> and purA<\/em> were removed from the chromosome of the receiving strain and therefore both substitutes can be selected. The precipitates prevent rearrangement between the valgenes and the chromosome and the formation of spontaneous Trp+<\/sup> and Ade+<\/sup> reversals. <\/p>\n\n\n\n

A small plexiglass, pRM21, was isolated from R. marinus<\/em> and sequentially. It consists of 2935 base pairs and its largest reading frame records for proteins that correspond to the replication proteins of large plasmids of the IncW family. The plasmid was used in genetic engineering experiments. A good transformation was obtained by electrolyzing the bacterium. The plasmid was also used as a basis for the construction of shuttle boats that replicate both R. marinus<\/em> and Escherichia coli. <\/em> Ferries were constructed for the expression of foreign genes in R. marinus<\/em> and increased protein production was obtained by using thermodynamic control sequences. Vices that also make it possible to study expression were also found R. marinus<\/em> and its stewards.<\/em><\/p>\n\n\n\n

Methods for inactivating genes in the genome were developed R. marinus,<\/em> both by random and targeted mutations. Genes were deleted from the bacterium chromosome without leaving any foreign sequences. Chromosome genes were also successfully replaced by double rearrangement by linear molecules. Induction of genetic modification R. marinus<\/em> opens up the possibility of research into its properties as well as utilization. Such methods are even more important now than before since the sequencing of the genome R. marinus<\/em> was recently published.<\/p>\n\n\n\n

Starts: 27\/09\/2010 - 13:00
Location: Main building<\/a>
Further location: Celebration hall<\/p>","protected":false},"excerpt":{"rendered":"

M\u00e1nudaginn 27. september mun starfsma\u00f0ur Mat\u00eds, Sn\u00e6d\u00eds Huld Bj\u00f6rnsd\u00f3ttir, verja doktorsritger\u00f0 s\u00edna \u201eErf\u00f0abreytingar \u00e1 bakter\u00edunni Rhodothermus marinus\u201c (e. Genetic engineering of Rhodothermus marinus). […]<\/p>\n","protected":false},"author":1,"featured_media":40343,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"templates\/template-cover.php","format":"standard","meta":{"_acf_changed":false,"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[1973],"tags":[],"class_list":["post-42706","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-frettir"],"acf":[],"yoast_head":"\nDoktorsv\u00f6rn \u00ed l\u00edffr\u00e6\u00f0i: Erf\u00f0abreytingar \u00e1 bakter\u00edunni Rhodothermus marinus - Mat\u00eds<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/matis.is\/en\/frettir\/doktorsvorn-i-liffraedi-erfdabreytingar-a-bakteriunni-rhodothermus-marinus\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Doktorsv\u00f6rn \u00ed l\u00edffr\u00e6\u00f0i: Erf\u00f0abreytingar \u00e1 bakter\u00edunni Rhodothermus marinus - Mat\u00eds\" \/>\n<meta property=\"og:description\" content=\"M\u00e1nudaginn 27. september mun starfsma\u00f0ur Mat\u00eds, Sn\u00e6d\u00eds Huld Bj\u00f6rnsd\u00f3ttir, verja doktorsritger\u00f0 s\u00edna \u201eErf\u00f0abreytingar \u00e1 bakter\u00edunni Rhodothermus marinus\u201c (e. 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