{"id":50216,"date":"2023-08-01T13:36:07","date_gmt":"2023-08-01T13:36:07","guid":{"rendered":"https:\/\/matis.is\/?p=50216"},"modified":"2023-08-01T13:38:57","modified_gmt":"2023-08-01T13:38:57","slug":"crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6","status":"publish","type":"post","link":"https:\/\/matis.is\/en\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/","title":{"rendered":"Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6"},"content":{"rendered":"<p>This study describes the production, characterization and structure determination of a novel Holliday junction-resolving enzyme. The enzyme, termed Hjc_15-6, is encoded in the genome of phage Tth15-6, which infects Thermus thermophilus. Hjc_15-6 was heterologously produced in Escherichia coli and high yields of soluble and biologically active recombinant enzyme were obtained in both complex and defined media. Amino-acid sequence and structure comparison suggested that the enzyme belongs to a group of enzymes classified as archaeal Holliday junction-resolving enzymes, which are typically divalent metal ion-binding dimers that are able to cleave X-shaped dsDNA-Holliday junctions (Hjs) . The crystal structure of Hjc_15-6 was determined to 2.5 \u00c5 resolution using the selenomethionine single-wavelength anomalous dispersion method. To our knowledge, this is the first crystal structure of an Hj-resolving enzyme originating from a bacteriophage that can be classified as an archaeal type of Hj-resolving enzyme. As such, it represents a new fold for Hj-resolving enzymes from phages. Characterization of the structure of Hjc_15-6 suggests that it may form a dimer, or even a homodimer of dimers, and activity studies show endonuclease activity towards Hjs. Furthermore, based on sequence analysis it is proposed that Hjc_15-6 has a three-part catalytic motif corresponding to E-SD-EVK, and this motif may be common among other Hj-resolving enzymes originating from thermophilic bacteriophages.<\/p>","protected":false},"excerpt":{"rendered":"<p>This study describes the production, characterization and structure determination of a novel Holliday junction-resolving enzyme. The enzyme, termed Hjc_15-6, is [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"template-greinar.php","format":"standard","meta":{"_acf_changed":false,"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[2011],"tags":[2147,2146,2148,2149],"class_list":["post-50216","post","type-post","status-publish","format-standard","hentry","category-greinar","tag-archaeal-like-holliday-junction-resolving-enzymes","tag-archaeal-like-holliday-junction-resolving-enzymes-signature-motifs-structure-function-relationship-thermophilic-bacteriophages","tag-signature-motifs","tag-thermophilic-bacteriophages"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6 - Mat\u00eds<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/matis.is\/en\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6 - Mat\u00eds\" \/>\n<meta property=\"og:description\" content=\"This study describes the production, characterization and structure determination of a novel Holliday junction-resolving enzyme. The enzyme, termed Hjc_15-6, is [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/matis.is\/en\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/\" \/>\n<meta property=\"og:site_name\" content=\"Mat\u00eds\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/matisiceland\" \/>\n<meta property=\"article:published_time\" content=\"2023-08-01T13:36:07+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-08-01T13:38:57+00:00\" \/>\n<meta name=\"author\" content=\"Krist\u00edn Edda Gylfad\u00f3ttir\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@matisiceland\" \/>\n<meta name=\"twitter:site\" content=\"@matisiceland\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Krist\u00edn Edda Gylfad\u00f3ttir\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/\"},\"author\":{\"name\":\"Krist\u00edn Edda Gylfad\u00f3ttir\",\"@id\":\"https:\/\/matis.is\/#\/schema\/person\/af2d479c72e5e79c4570d409abf2dc9b\"},\"headline\":\"Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6\",\"datePublished\":\"2023-08-01T13:36:07+00:00\",\"dateModified\":\"2023-08-01T13:38:57+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/\"},\"wordCount\":232,\"publisher\":{\"@id\":\"https:\/\/matis.is\/#organization\"},\"keywords\":[\"archaeal-like Holliday junction-resolving enzymes\",\"archaeal-like Holliday junction-resolving enzymes; signature motifs; structure\u2013function relationship; thermophilic bacteriophages\",\"signature motifs\",\"thermophilic bacteriophages\"],\"articleSection\":[\"Greinar\"],\"inLanguage\":\"en-GB\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/\",\"url\":\"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/\",\"name\":\"Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6 - Mat\u00eds\",\"isPartOf\":{\"@id\":\"https:\/\/matis.is\/#website\"},\"datePublished\":\"2023-08-01T13:36:07+00:00\",\"dateModified\":\"2023-08-01T13:38:57+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/matis.is\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/matis.is\/#website\",\"url\":\"https:\/\/matis.is\/\",\"name\":\"Mat\u00eds\",\"description\":\"Ver\u00f0m\u00e6task\u00f6pun, matv\u00e6la\u00f6ryggi og l\u00fd\u00f0heilsa\",\"publisher\":{\"@id\":\"https:\/\/matis.is\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/matis.is\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-GB\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/matis.is\/#organization\",\"name\":\"Mat\u00eds ohf.\",\"url\":\"https:\/\/matis.is\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\/\/matis.is\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png\",\"contentUrl\":\"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png\",\"width\":1507,\"height\":1507,\"caption\":\"Mat\u00eds ohf.\"},\"image\":{\"@id\":\"https:\/\/matis.is\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/www.facebook.com\/matisiceland\",\"https:\/\/x.com\/matisiceland\",\"https:\/\/www.instagram.com\/matisiceland\/\",\"https:\/\/www.linkedin.com\/matisiceland\/\",\"https:\/\/www.youtube.com\/channel\/UCY-frt3uTqgVZW-DLjoo5bA\"]},{\"@type\":\"Person\",\"@id\":\"https:\/\/matis.is\/#\/schema\/person\/af2d479c72e5e79c4570d409abf2dc9b\",\"name\":\"Krist\u00edn Edda Gylfad\u00f3ttir\",\"url\":\"https:\/\/matis.is\/en\/author\/kristingmatis-is\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6 - Mat\u00eds","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/matis.is\/en\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/","og_locale":"en_GB","og_type":"article","og_title":"Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6 - Mat\u00eds","og_description":"This study describes the production, characterization and structure determination of a novel Holliday junction-resolving enzyme. The enzyme, termed Hjc_15-6, is [&hellip;]","og_url":"https:\/\/matis.is\/en\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/","og_site_name":"Mat\u00eds","article_publisher":"https:\/\/www.facebook.com\/matisiceland","article_published_time":"2023-08-01T13:36:07+00:00","article_modified_time":"2023-08-01T13:38:57+00:00","author":"Krist\u00edn Edda Gylfad\u00f3ttir","twitter_card":"summary_large_image","twitter_creator":"@matisiceland","twitter_site":"@matisiceland","twitter_misc":{"Written by":"Krist\u00edn Edda Gylfad\u00f3ttir","Estimated reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/#article","isPartOf":{"@id":"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/"},"author":{"name":"Krist\u00edn Edda Gylfad\u00f3ttir","@id":"https:\/\/matis.is\/#\/schema\/person\/af2d479c72e5e79c4570d409abf2dc9b"},"headline":"Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6","datePublished":"2023-08-01T13:36:07+00:00","dateModified":"2023-08-01T13:38:57+00:00","mainEntityOfPage":{"@id":"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/"},"wordCount":232,"publisher":{"@id":"https:\/\/matis.is\/#organization"},"keywords":["archaeal-like Holliday junction-resolving enzymes","archaeal-like Holliday junction-resolving enzymes; signature motifs; structure\u2013function relationship; thermophilic bacteriophages","signature motifs","thermophilic bacteriophages"],"articleSection":["Greinar"],"inLanguage":"en-GB"},{"@type":"WebPage","@id":"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/","url":"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/","name":"Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6 - Mat\u00eds","isPartOf":{"@id":"https:\/\/matis.is\/#website"},"datePublished":"2023-08-01T13:36:07+00:00","dateModified":"2023-08-01T13:38:57+00:00","breadcrumb":{"@id":"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/#breadcrumb"},"inLanguage":"en-GB","potentialAction":[{"@type":"ReadAction","target":["https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/matis.is\/greinar\/crystal-structure-and-initial-characterization-of-a-novel-archaeal-like-holliday-junction-resolving-enzyme-from-thermus-thermophilus-phage-tth15-6\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/matis.is\/"},{"@type":"ListItem","position":2,"name":"Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6"}]},{"@type":"WebSite","@id":"https:\/\/matis.is\/#website","url":"https:\/\/matis.is\/","name":"Mat\u00eds","description":"Value creation, food security and public health","publisher":{"@id":"https:\/\/matis.is\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/matis.is\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-GB"},{"@type":"Organization","@id":"https:\/\/matis.is\/#organization","name":"Mat\u00eds ohf.","url":"https:\/\/matis.is\/","logo":{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/matis.is\/#\/schema\/logo\/image\/","url":"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png","contentUrl":"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png","width":1507,"height":1507,"caption":"Mat\u00eds ohf."},"image":{"@id":"https:\/\/matis.is\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/matisiceland","https:\/\/x.com\/matisiceland","https:\/\/www.instagram.com\/matisiceland\/","https:\/\/www.linkedin.com\/matisiceland\/","https:\/\/www.youtube.com\/channel\/UCY-frt3uTqgVZW-DLjoo5bA"]},{"@type":"Person","@id":"https:\/\/matis.is\/#\/schema\/person\/af2d479c72e5e79c4570d409abf2dc9b","name":"Krist\u00edn Edda Gylfad\u00f3ttir","url":"https:\/\/matis.is\/en\/author\/kristingmatis-is\/"}]}},"spectra_custom_meta":{"_edit_lock":["1690897009:3"],"_wp_page_template":["template-greinar.php"],"_edit_last":["3"],"_yoast_wpseo_content_score":["30"],"_yoast_wpseo_estimated-reading-time-minutes":["2"],"_yoast_wpseo_wordproof_timestamp":[""],"authors":["Josefin Ahlqvist, Javier A Linares-Past\u00e9n, Maria H\u00e5kansson, Andrius Jasilionis, Karolina Kwiatkowska-Semrau, \u00d3lafur H Fri\u00f0j\u00f3nsson, Anna Karina Kaczorowska, Slawomir Dabrowski, Arn\u00fe\u00f3r \u00c6varsson, Gu\u00f0mundur \u00d3 Hreggvi\u00f0sson, Salam Al-Karadaghi, Tadeusz Kaczorowski, Eva Nordberg Karlsson"],"_authors":["field_60a287218a0ab"],"publication":["Acta Crystallogr D Struct Biol"],"_publication":["field_60a287738a0ac"],"edition":["Feb 1;78(Pt 2):212-227"],"_edition":["field_60a287918a0ad"],"page":["212-227"],"_page":["field_60a2879f8a0ae"],"year":["2022"],"_year":["field_60a287a58a0af"],"link":["a:3:{s:5:\"title\";s:0:\"\";s:3:\"url\";s:41:\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35102887\/\";s:6:\"target\";s:0:\"\";}"],"_link":["field_60a287aa8a0b0"],"orcidid":[""],"_orcidid":["field_60a287c28a0b1"],"matis_tengilidur":["a:1:{i:0;s:5:\"28263\";}"],"_matis_tengilidur":["field_60a65b61f4532"],"tengill_a_grein":["https:\/\/pubmed.ncbi.nlm.nih.gov\/35102887\/"],"_tengill_a_grein":["field_61641790ac6c9"],"doi":["10.1107\/S2059798321012298"],"_doi":["field_616417d8ac6ca"],"_":["field_630df27cc5801"],"tengilidur":["28263"],"_tengilidur":["field_5ef905e0538b2"],"_yoast_wpseo_primary_category":["2011"],"wpmm_postgrid_views":["308"],"_uag_css_file_name":["uag-css-50216.css"],"_uag_page_assets":["a:9:{s:3:\"css\";s:0:\"\";s:2:\"js\";s:0:\"\";s:18:\"current_block_list\";a:6:{i:0;s:14:\"core\/paragraph\";i:1;s:12:\"core\/buttons\";i:2;s:11:\"core\/button\";i:3;s:12:\"core\/heading\";i:4;s:12:\"core\/columns\";i:5;s:11:\"core\/column\";}s:8:\"uag_flag\";b:0;s:11:\"uag_version\";s:10:\"1775648310\";s:6:\"gfonts\";a:0:{}s:10:\"gfonts_url\";s:0:\"\";s:12:\"gfonts_files\";a:0:{}s:14:\"uag_faq_layout\";b:0;}"]},"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false},"uagb_author_info":{"display_name":"Krist\u00edn Edda Gylfad\u00f3ttir","author_link":"https:\/\/matis.is\/en\/author\/kristingmatis-is\/"},"uagb_comment_info":0,"uagb_excerpt":"This study describes the production, characterization and structure determination of a novel Holliday junction-resolving enzyme. The enzyme, termed Hjc_15-6, is [&hellip;]","_links":{"self":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts\/50216","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/comments?post=50216"}],"version-history":[{"count":5,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts\/50216\/revisions"}],"predecessor-version":[{"id":50221,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts\/50216\/revisions\/50221"}],"wp:attachment":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/media?parent=50216"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/categories?post=50216"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/tags?post=50216"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}