{"id":50222,"date":"2023-08-01T13:47:40","date_gmt":"2023-08-01T13:47:40","guid":{"rendered":"https:\/\/matis.is\/?p=50222"},"modified":"2023-08-01T13:47:42","modified_gmt":"2023-08-01T13:47:42","slug":"crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c","status":"publish","type":"post","link":"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/","title":{"rendered":"Crystal structure of DNA polymerase I from\u00a0<em>Thermus<\/em>\u00a0phage G20c"},"content":{"rendered":"<p>This study describes the structure of DNA polymerase I from\u00a0<em>Thermus<\/em>\u00a0phage G20c, termed PolI_G20c. This is the first structure of a DNA polymerase originating from a group of related thermophilic bacteriophages infecting\u00a0<em>Thermus thermophilus<\/em>, including phages G20c, TSP4, P74-26, P23-45 and phiFA and the novel phage Tth15-6. Sequence and structural analysis of PolI_G20c revealed a 3\u2032\u20135\u2032 exonuclease domain and a DNA polymerase domain, and activity screening confirmed that both domains were functional. No functional 5\u2032\u20133\u2032 exonuclease domain was present. Structural analysis also revealed a novel specific structural motif, here termed S\u03b2\u03b1R, that was not previously identified in any polymerase belonging to the DNA polymerases I (or the DNA polymerase A family). The S\u03b2\u03b1R motif did not show any homology to the sequences or structures of known DNA polymerases. The exception was the sequence conservation of the residues in this motif in putative DNA polymerases encoded in the genomes of a group of thermophilic phages related to\u00a0<em>Thermus<\/em>\u00a0phage G20c. The structure of PolI_G20c was determined with the aid of another structure that was determined in parallel and was used as a model for molecular replacement. This other structure was of a 3\u2032\u20135\u2032 exonuclease termed ExnV1. The cloned and expressed gene encoding ExnV1 was isolated from a thermophilic virus metagenome that was collected from several hot springs in Iceland. The structure of ExnV1, which contains the novel S\u03b2\u03b1R motif, was first determined to 2.19 \u00c5 resolution. With these data at hand, the structure of PolI_G20c was determined to 2.97 \u00c5 resolution. The structures of PolI_G20c and ExnV1 are most similar to those of the Klenow fragment of DNA polymerase I (PDB entry\u00a0<a href=\"https:\/\/scripts.iucr.org\/cgi-bin\/cr.cgi?rm=pdb&amp;pdbId=2kzz\" target=\"_blank\" rel=\"noreferrer noopener\">2 kzz<\/a>) from\u00a0<em>Escherichia coli<\/em>, DNA polymerase I from\u00a0<em>Geobacillus stearothermophilus<\/em>\u00a0(PDB entry\u00a0<a href=\"https:\/\/scripts.iucr.org\/cgi-bin\/cr.cgi?rm=pdb&amp;pdbId=1knc\" target=\"_blank\" rel=\"noreferrer noopener\">1 knc<\/a>) and\u00a0<em>Taq<\/em>\u00a0polymerase (PDB entry\u00a0<a href=\"https:\/\/scripts.iucr.org\/cgi-bin\/cr.cgi?rm=pdb&amp;pdbId=1bgx\" target=\"_blank\" rel=\"noreferrer noopener\">1 bgx<\/a>) from\u00a0<em>Thermus aquaticus<\/em>.<\/p>","protected":false},"excerpt":{"rendered":"<p>This study describes the structure of DNA polymerase I from\u00a0Thermus\u00a0phage G20c, termed PolI_G20c. This is the first structure of a [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"template-greinar.php","format":"standard","meta":{"_acf_changed":false,"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[2011],"tags":[2150,2154,2153,2149,2151,2152],"class_list":["post-50222","post","type-post","status-publish","format-standard","hentry","category-greinar","tag-dna-polymerase-i","tag-poli_g20c","tag-structural-motifs","tag-thermophilic-bacteriophages","tag-thermus-phage-g20c","tag-thermus-thermophilus"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Crystal structure of DNA polymerase I from\u00a0Thermus\u00a0phage G20c - Mat\u00eds<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Crystal structure of DNA polymerase I from\u00a0Thermus\u00a0phage G20c - Mat\u00eds\" \/>\n<meta property=\"og:description\" content=\"This study describes the structure of DNA polymerase I from\u00a0Thermus\u00a0phage G20c, termed PolI_G20c. This is the first structure of a [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/\" \/>\n<meta property=\"og:site_name\" content=\"Mat\u00eds\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/matisiceland\" \/>\n<meta property=\"article:published_time\" content=\"2023-08-01T13:47:40+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-08-01T13:47:42+00:00\" \/>\n<meta name=\"author\" content=\"Krist\u00edn Edda Gylfad\u00f3ttir\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@matisiceland\" \/>\n<meta name=\"twitter:site\" content=\"@matisiceland\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Krist\u00edn Edda Gylfad\u00f3ttir\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/\"},\"author\":{\"name\":\"Krist\u00edn Edda Gylfad\u00f3ttir\",\"@id\":\"https:\/\/matis.is\/#\/schema\/person\/af2d479c72e5e79c4570d409abf2dc9b\"},\"headline\":\"Crystal structure of DNA polymerase I from\u00a0Thermus\u00a0phage G20c\",\"datePublished\":\"2023-08-01T13:47:40+00:00\",\"dateModified\":\"2023-08-01T13:47:42+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/\"},\"wordCount\":318,\"publisher\":{\"@id\":\"https:\/\/matis.is\/#organization\"},\"keywords\":[\"DNA polymerase I\",\"PolI_G20c\",\"structural motifs\",\"thermophilic bacteriophages\",\"Thermus phage G20c\",\"Thermus thermophilus\"],\"articleSection\":[\"Greinar\"],\"inLanguage\":\"en-GB\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/\",\"url\":\"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/\",\"name\":\"Crystal structure of DNA polymerase I from\u00a0Thermus\u00a0phage G20c - Mat\u00eds\",\"isPartOf\":{\"@id\":\"https:\/\/matis.is\/#website\"},\"datePublished\":\"2023-08-01T13:47:40+00:00\",\"dateModified\":\"2023-08-01T13:47:42+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/matis.is\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Crystal structure of DNA polymerase I from\u00a0Thermus\u00a0phage G20c\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/matis.is\/#website\",\"url\":\"https:\/\/matis.is\/\",\"name\":\"Mat\u00eds\",\"description\":\"Ver\u00f0m\u00e6task\u00f6pun, matv\u00e6la\u00f6ryggi og l\u00fd\u00f0heilsa\",\"publisher\":{\"@id\":\"https:\/\/matis.is\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/matis.is\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-GB\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/matis.is\/#organization\",\"name\":\"Mat\u00eds ohf.\",\"url\":\"https:\/\/matis.is\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\/\/matis.is\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png\",\"contentUrl\":\"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png\",\"width\":1507,\"height\":1507,\"caption\":\"Mat\u00eds ohf.\"},\"image\":{\"@id\":\"https:\/\/matis.is\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/www.facebook.com\/matisiceland\",\"https:\/\/x.com\/matisiceland\",\"https:\/\/www.instagram.com\/matisiceland\/\",\"https:\/\/www.linkedin.com\/matisiceland\/\",\"https:\/\/www.youtube.com\/channel\/UCY-frt3uTqgVZW-DLjoo5bA\"]},{\"@type\":\"Person\",\"@id\":\"https:\/\/matis.is\/#\/schema\/person\/af2d479c72e5e79c4570d409abf2dc9b\",\"name\":\"Krist\u00edn Edda Gylfad\u00f3ttir\",\"url\":\"https:\/\/matis.is\/en\/author\/kristingmatis-is\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Crystal structure of DNA polymerase I from Thermus phage G20c - Mat\u00eds","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/","og_locale":"en_GB","og_type":"article","og_title":"Crystal structure of DNA polymerase I from\u00a0Thermus\u00a0phage G20c - Mat\u00eds","og_description":"This study describes the structure of DNA polymerase I from\u00a0Thermus\u00a0phage G20c, termed PolI_G20c. This is the first structure of a [&hellip;]","og_url":"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/","og_site_name":"Mat\u00eds","article_publisher":"https:\/\/www.facebook.com\/matisiceland","article_published_time":"2023-08-01T13:47:40+00:00","article_modified_time":"2023-08-01T13:47:42+00:00","author":"Krist\u00edn Edda Gylfad\u00f3ttir","twitter_card":"summary_large_image","twitter_creator":"@matisiceland","twitter_site":"@matisiceland","twitter_misc":{"Written by":"Krist\u00edn Edda Gylfad\u00f3ttir","Estimated reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/#article","isPartOf":{"@id":"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/"},"author":{"name":"Krist\u00edn Edda Gylfad\u00f3ttir","@id":"https:\/\/matis.is\/#\/schema\/person\/af2d479c72e5e79c4570d409abf2dc9b"},"headline":"Crystal structure of DNA polymerase I from\u00a0Thermus\u00a0phage G20c","datePublished":"2023-08-01T13:47:40+00:00","dateModified":"2023-08-01T13:47:42+00:00","mainEntityOfPage":{"@id":"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/"},"wordCount":318,"publisher":{"@id":"https:\/\/matis.is\/#organization"},"keywords":["DNA polymerase I","PolI_G20c","structural motifs","thermophilic bacteriophages","Thermus phage G20c","Thermus thermophilus"],"articleSection":["Greinar"],"inLanguage":"en-GB"},{"@type":"WebPage","@id":"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/","url":"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/","name":"Crystal structure of DNA polymerase I from Thermus phage G20c - Mat\u00eds","isPartOf":{"@id":"https:\/\/matis.is\/#website"},"datePublished":"2023-08-01T13:47:40+00:00","dateModified":"2023-08-01T13:47:42+00:00","breadcrumb":{"@id":"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/#breadcrumb"},"inLanguage":"en-GB","potentialAction":[{"@type":"ReadAction","target":["https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/matis.is\/en\/greinar\/crystal-structure-of-dna-polymerase-i-from-thermus-phage-g20c\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/matis.is\/"},{"@type":"ListItem","position":2,"name":"Crystal structure of DNA polymerase I from\u00a0Thermus\u00a0phage G20c"}]},{"@type":"WebSite","@id":"https:\/\/matis.is\/#website","url":"https:\/\/matis.is\/","name":"Mat\u00eds","description":"Value creation, food security and public health","publisher":{"@id":"https:\/\/matis.is\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/matis.is\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-GB"},{"@type":"Organization","@id":"https:\/\/matis.is\/#organization","name":"Mat\u00eds ohf.","url":"https:\/\/matis.is\/","logo":{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/matis.is\/#\/schema\/logo\/image\/","url":"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png","contentUrl":"https:\/\/matis.is\/wp-content\/uploads\/2021\/02\/matis_logo_blatthvitt.png","width":1507,"height":1507,"caption":"Mat\u00eds ohf."},"image":{"@id":"https:\/\/matis.is\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/matisiceland","https:\/\/x.com\/matisiceland","https:\/\/www.instagram.com\/matisiceland\/","https:\/\/www.linkedin.com\/matisiceland\/","https:\/\/www.youtube.com\/channel\/UCY-frt3uTqgVZW-DLjoo5bA"]},{"@type":"Person","@id":"https:\/\/matis.is\/#\/schema\/person\/af2d479c72e5e79c4570d409abf2dc9b","name":"Krist\u00edn Edda Gylfad\u00f3ttir","url":"https:\/\/matis.is\/en\/author\/kristingmatis-is\/"}]}},"spectra_custom_meta":{"_edit_lock":["1690897663:3"],"_wp_page_template":["template-greinar.php"],"_edit_last":["3"],"_yoast_wpseo_content_score":["30"],"_yoast_wpseo_estimated-reading-time-minutes":["2"],"_yoast_wpseo_wordproof_timestamp":[""],"authors":["Josefin Ahlqvist, Javier A. Linares-Past\u00e9n, Andrius Jasilionis, Martin Welin, Maria H\u00e5kansson, L. Anders Svensson, Lei Wang, Hildegard Watzlawick, Arn\u00fe\u00f3r \u00c6varsson, \u00d3lafur H. Fri\u00f0j\u00f3nsson, Gu\u00f0mundur \u00d3. Hreggvi\u00f0sson, Bernd Ketelsen Striberny, Eirin Glomsaker, Olav Lanes, Salam Al-Karadaghi and Eva Nordberg Karlsson"],"_authors":["field_60a287218a0ab"],"publication":["Acta Crystallogr D Struct Biol"],"_publication":["field_60a287738a0ac"],"edition":["Nov 1; 78(Pt 11)"],"_edition":["field_60a287918a0ad"],"page":["1384\u20131398"],"_page":["field_60a2879f8a0ae"],"year":["2022"],"_year":["field_60a287a58a0af"],"link":["a:3:{s:5:\"title\";s:0:\"\";s:3:\"url\";s:53:\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9629493\/\";s:6:\"target\";s:0:\"\";}"],"_link":["field_60a287aa8a0b0"],"orcidid":[""],"_orcidid":["field_60a287c28a0b1"],"matis_tengilidur":["a:1:{i:0;s:5:\"28169\";}"],"_matis_tengilidur":["field_60a65b61f4532"],"tengill_a_grein":["https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9629493\/"],"_tengill_a_grein":["field_61641790ac6c9"],"doi":["10.1107\/S2059798322009895"],"_doi":["field_616417d8ac6ca"],"_":["field_630df27cc5801"],"tengilidur":["28169"],"_tengilidur":["field_5ef905e0538b2"],"_yoast_wpseo_primary_category":["2011"],"wpmm_postgrid_views":["376"],"_uag_css_file_name":["uag-css-50222.css"],"_uag_page_assets":["a:9:{s:3:\"css\";s:263:\".uag-blocks-common-selector{z-index:var(--z-index-desktop) !important}@media (max-width: 976px){.uag-blocks-common-selector{z-index:var(--z-index-tablet) !important}}@media (max-width: 767px){.uag-blocks-common-selector{z-index:var(--z-index-mobile) !important}}\n\";s:2:\"js\";s:0:\"\";s:18:\"current_block_list\";a:6:{i:0;s:14:\"core\/paragraph\";i:1;s:12:\"core\/buttons\";i:2;s:11:\"core\/button\";i:3;s:12:\"core\/heading\";i:4;s:12:\"core\/columns\";i:5;s:11:\"core\/column\";}s:8:\"uag_flag\";b:0;s:11:\"uag_version\";s:10:\"1775648310\";s:6:\"gfonts\";a:0:{}s:10:\"gfonts_url\";s:0:\"\";s:12:\"gfonts_files\";a:0:{}s:14:\"uag_faq_layout\";b:0;}"]},"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false},"uagb_author_info":{"display_name":"Krist\u00edn Edda Gylfad\u00f3ttir","author_link":"https:\/\/matis.is\/en\/author\/kristingmatis-is\/"},"uagb_comment_info":0,"uagb_excerpt":"This study describes the structure of DNA polymerase I from\u00a0Thermus\u00a0phage G20c, termed PolI_G20c. This is the first structure of a [&hellip;]","_links":{"self":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts\/50222","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/comments?post=50222"}],"version-history":[{"count":3,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts\/50222\/revisions"}],"predecessor-version":[{"id":50225,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/posts\/50222\/revisions\/50225"}],"wp:attachment":[{"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/media?parent=50222"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/categories?post=50222"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/matis.is\/en\/wp-json\/wp\/v2\/tags?post=50222"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}