{"id":50303,"date":"2023-08-02T09:29:36","date_gmt":"2023-08-02T09:29:36","guid":{"rendered":"https:\/\/matis.is\/?p=50303"},"modified":"2023-08-02T09:29:37","modified_gmt":"2023-08-02T09:29:37","slug":"protein-characteristics-and-bioactivity-of-fish-protein-hydrolysates-from-tra-catfish-pangasius-hypophthalmus-side-stream-isolates","status":"publish","type":"post","link":"https:\/\/matis.is\/en\/greinar\/protein-characteristics-and-bioactivity-of-fish-protein-hydrolysates-from-tra-catfish-pangasius-hypophthalmus-side-stream-isolates\/","title":{"rendered":"Protein Characteristics and Bioactivity of Fish Protein Hydrolysates from Tra Catfish (<em>Pangasius hypophthalmus<\/em>) Side Stream Isolates"},"content":{"rendered":"<p>Enzymatic hydrolysis is a novel method to recover highly potent bioactive fish protein hydrolysates (FPHs) from fish processing side-streams. The common way of producing FPHs directly from fish side-streams may be inappropriate due to the excess of lipids and pro-oxidants, especially in lipid-rich streams, as obtained from Tra catfish. This study aimed to optimize the hydrolysis conditions for a commercial enzyme (Alcalase\u00ae 2.4 L) (enzyme concentrate, temperature, and time) in FPH production from the fish protein isolate obtained from Tra catfish dark muscle (DM-FPI) using the pH- shift method. The degree of hydrolysis (DH), protein recovery (PR), and antioxidant properties, including DPPH radical scavenging activity (DPPH-RSA) and total reducing power capacity (TRPC), were measured to evaluate the effects of the hydrolysis conditions on the FPHs . Optimal hydrolysis was obtained at an enzyme\/substrate protein ratio of 3% (<em>v<\/em>\/<em>w<\/em>) and a hydrolysis temperature of 50 \u00b0C for 3 h. The FPHs obtained from different substrates, including DM-FPI, abdominal cut-off (ACO) FPI, and head and backbone blend (HBB) FPI, had similar DHs under these optimum conditions, ranging from 22.5% to 24.0%. However, the FPH obtained from abdominal cut-off isolate (ACO-FPH) showed the highest PR of 81.5 \u00b1 4.3% and the highest antioxidant properties, with a DPPH-RSA of 86.1 \u00b1 1.6% and a TRPC of 6.4 \u00b1 0.4 equivalent mg vitamin C\/g protein. The resulting FPHs present a natural source of antioxidants with great potential for food applications, especially the ACO-FPH. In addition, all FPHs had excellent amino acid profiles, indicating strong potential for their use as supplements. Tra catfish protein-rich side-streams can thus be processed into high-value bioactive FPHs using Alcalase for human consumption.<\/p>","protected":false},"excerpt":{"rendered":"<p>Enzymatic hydrolysis is a novel method to recover highly potent bioactive fish protein hydrolysates (FPHs) from fish processing side-streams. The [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"template-greinar.php","format":"standard","meta":{"_acf_changed":false,"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[2011],"tags":[],"class_list":["post-50303","post","type-post","status-publish","format-standard","hentry","category-greinar"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Protein Characteristics and Bioactivity of Fish Protein Hydrolysates from Tra Catfish (Pangasius hypophthalmus) Side Stream Isolates - Mat\u00eds<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/matis.is\/en\/greinar\/protein-characteristics-and-bioactivity-of-fish-protein-hydrolysates-from-tra-catfish-pangasius-hypophthalmus-side-stream-isolates\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Protein Characteristics and Bioactivity of Fish Protein Hydrolysates from Tra Catfish (Pangasius hypophthalmus) Side Stream Isolates - Mat\u00eds\" \/>\n<meta property=\"og:description\" content=\"Enzymatic hydrolysis is a novel method to recover highly potent bioactive fish protein hydrolysates (FPHs) from fish processing side-streams. The [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/matis.is\/en\/greinar\/protein-characteristics-and-bioactivity-of-fish-protein-hydrolysates-from-tra-catfish-pangasius-hypophthalmus-side-stream-isolates\/\" \/>\n<meta property=\"og:site_name\" content=\"Mat\u00eds\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/matisiceland\" \/>\n<meta property=\"article:published_time\" content=\"2023-08-02T09:29:36+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-08-02T09:29:37+00:00\" \/>\n<meta name=\"author\" content=\"Krist\u00edn Edda Gylfad\u00f3ttir\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@matisiceland\" \/>\n<meta name=\"twitter:site\" content=\"@matisiceland\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Krist\u00edn Edda Gylfad\u00f3ttir\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/matis.is\/en\/greinar\/protein-characteristics-and-bioactivity-of-fish-protein-hydrolysates-from-tra-catfish-pangasius-hypophthalmus-side-stream-isolates\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/matis.is\/en\/greinar\/protein-characteristics-and-bioactivity-of-fish-protein-hydrolysates-from-tra-catfish-pangasius-hypophthalmus-side-stream-isolates\/\"},\"author\":{\"name\":\"Krist\u00edn Edda Gylfad\u00f3ttir\",\"@id\":\"https:\/\/matis.is\/#\/schema\/person\/af2d479c72e5e79c4570d409abf2dc9b\"},\"headline\":\"Protein Characteristics and Bioactivity of Fish Protein Hydrolysates from Tra Catfish (Pangasius hypophthalmus) Side Stream Isolates\",\"datePublished\":\"2023-08-02T09:29:36+00:00\",\"dateModified\":\"2023-08-02T09:29:37+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/matis.is\/en\/greinar\/protein-characteristics-and-bioactivity-of-fish-protein-hydrolysates-from-tra-catfish-pangasius-hypophthalmus-side-stream-isolates\/\"},\"wordCount\":274,\"publisher\":{\"@id\":\"https:\/\/matis.is\/#organization\"},\"articleSection\":[\"Greinar\"],\"inLanguage\":\"en-GB\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/matis.is\/en\/greinar\/protein-characteristics-and-bioactivity-of-fish-protein-hydrolysates-from-tra-catfish-pangasius-hypophthalmus-side-stream-isolates\/\",\"url\":\"https:\/\/matis.is\/en\/greinar\/protein-characteristics-and-bioactivity-of-fish-protein-hydrolysates-from-tra-catfish-pangasius-hypophthalmus-side-stream-isolates\/\",\"name\":\"Protein Characteristics and Bioactivity of Fish Protein Hydrolysates from Tra Catfish (Pangasius hypophthalmus) Side Stream Isolates - 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