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Determination of inorganic arsenic in seafood: Emphasizing the need for certified reference materials

Höfundar: Ásta Heiðrún Pétursdóttir, Helga Gunnlaugsdóttir, Hrönn Jörundsdóttir, Andrea Raab, Eva M. Krupp, Jörg Feldmann

Útgáfa: Pure and Applied Chemistry

Útgáfuár: 2012


o evaluate the accuracy and robustness of an extraction method, utilizing an alkaline-ethanolic solution and microwave heating, the certified reference material (CRM)TORT-2 was subjected to three different instrumental methodologies: high-performance liq-uid chromatography (HPLC), coupled with and without post-column hydride generation;inductively coupled plasma-mass spectrometry (ICP-MS); and HPLC-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS). The three methods gave a consistentvalue of inorganic arsenic (As) which is near the mean value of the reported values in the lit-erature, which, however, range by a factor of 10. Inorganic As, defined here as all As speciesthat do not have an As–C bond, that is, the sum of arsenite and arsenate and any thiol-boundAs, was found to be less than 4 % of total As concentration in 12 samples of fish meal whensubjected to this extraction method followed by HPLC-ICP-MS. To date, there is no certifiedvalue of inorganic As in a seafood-based reference material to compare to in order to vali-date the findings. This illustrates the difficulties in quantitative determination of inorganic Asin seafood and the need for a reference material for inorganic As and proficiency tests inorder to introduce legislation for a maximum level of inorganic As in seafood and feed.

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