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Proteome analysis of abundant proteins in early Atlantic cod (Gadus morhua) larvae

Höfundar: Holmfridur Sveinsdottir, Agusta Gudmundsdottir

Útgáfa: Icelandic Agricultural Sciences

Útgáfuár: 2011

Samantekt:

The production of good quality larvae is a challenge in marine fish hatcheries. In this study, the proteome profile of Atlantic cod (Gadus morhua) was analyzed using 2-DE, MALDI TOF MS and LC-MS/MS in order to determine its protein composition. Out of 109 abundant spots analyzed with MS 77 proteins were identified. The identified proteins were classified into 5 groups: structural proteins (56%), cytosolic proteins (29%), mitochondrial proteins (7%), secreted and extracellular proteins (7%) and nuclear proteins (1%). This study is the first step in a future construction of an Atlantic cod protein database that will be a valuable resource for molecular analysis of marine fish larval development.

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