Fish anti-diabetic fish proteins
The purpose of this project was to screen for the bioactivity of peptides produced from three different fish species: cod (Gadus morhua), saithe (Pollachius virens) and char (Salvelinus alpinus). The proteins were hydrolyzed with four types of proteolytic enzymes, viz. Alcalase and Protamex from Novozyme and Protease M and Protease P from Amano enzyme, and their bioactivity studied. The peptides were also digested with digestive fluids containing digestive enzymes to examine whether digestion affected their bioactivity. The aim of the project was also to investigate the anti-diabetic effect of the peptides through chemical and cellular tests. The main results indicate that the peptides have a positive health effect, especially antioxidant activity and antihypertensive activity. At the same time, the antioxidant activity increased after digestion. It was difficult to measure the anti-diabetic effect of the peptides by cellular tests and it is necessary to develop this method better for further research on the anti-diabetic effect of bioactive substances. Overall, the project is an important part of acquiring knowledge of the composition and properties of fish peptides extracted from different raw materials with different enzymes. The methods that were set up will be used in the future to analyze and verify further bioactivity in products made from Icelandic seafood.
This AVS project was primarily aimed at screen for the peptide characteristics of three different fish species: Atlantic cod (Gadus morhua), saithe (Pollachius virens) and Artic charr (Salvelinus alpinus). The proteins were hydrolyzed using four different proteolytic enzymes: Alcalase and Protamex from Novozyme and Protease M and Protease P from Amano enzyme by measuring the bioactive properties of these peptides. Moreover, after analyzing the characteristics of these peptides, the goal was to apply simulated gastrointestinal digestion and compare the digested peptides to undigested peptides to see if there were increases in bioactivities. Finally, this project also focused on the anti ‐ diabetes properties by using in ‐ vitro chemical based assays and in ‐ vitro cellular based assays. In general, the project results indicate possible health benefits of the fish peptides, particularly good anti ‐ oxidant and anti ‐ hypertensive effects. Interestingly, there was an increase of antioxidant properties after applying simulated gastrointestinal digestion to the hydrolysates. However, cell ‐ based assays testing anti ‐ diabetes effects proved to be very challenging while chemical in ‐ vitro test did not show any anti ‐ diabetes properties of the fish hydrolysates. We suggest that more research efforts need to be directed towards the development of assays measuring anti ‐ diabetes effects.
Report closed until 01.10.2015