Reports

TOPCOD, OPTILAR / Long live the first model. Preferred breeding processes in larval and juvenile farming of cod

Published:

01/04/2011

Authors:

Jónína Jóhannsdóttir, Agnar Steinarsson, Rannveig Björnsdóttir

Supported by:

Technology Development Fund and AVS R&D Fund of Ministry of Fisheries in Iceland

TOPCOD, OPTILAR / Long live the first model. Preferred breeding processes in larval and juvenile farming of cod

Recent research has shown that the best conditions for the production of larvae produce juveniles of better quality and that the growth advantage in the early stages of rearing pays off to some extent in the later stages of growth. The main goal of this project is to define the best conditions for cod cod farming in Iceland and to that end use various methods to solve the main problems associated with the production of cod larvae today. This report discusses the work components that Matís ohf. participated in, among other things, researching the effects of enrichment of feed animals with supplementary bacteria and protein digestion on the growth, development, immune stimulation and digestive flora of larvae, as well as research on the effects of different primary feeds on muscle growth carried out in collaboration with the MRI. The results indicate that the start of dry feed feeding late or around 50 dph does not give the larvae a growth advantage and that it is sufficient to feed Artemia up to 40 dph. Dry feed from 30 dph led to lower growth and an increased incidence of structural defects. Enrichment of feed animals with a lyophilized mixture of two complementary bacterial strains did not affect the composition of bacterial flora larvae and strains did not gain a foothold in treated larvae. However, poor roe quality can be expected to have affected treatment outcomes. The results of experiments confirm previous results on the positive effect of enrichment of feed animals with protein digestion on the performance and development of larvae.  

Recent research has demonstrated that production optimization during the larval and juvenile phase will to some extent be reflected in the performance of the fish during the ongrowing phase. The objectives of the project are to optimize the larval production of Atlantic cod in Iceland by applying a multidisciplinary approach to solve central bottlenecks related to larval production. This report presents tasks where Matis ohf. was involved, including analyzes of the effects of live prey enrichment using putative probionts and a fish protein hydrolyzate on larval survival quality immune stimulation and intestinal bacterial community of larvae. The study also involved an analysis of the effect of startfeeding protocols on muscle growth in collaboration with MRI. The results indicate that late weaning around 50 dph may be excessive and produce no significant advantage. An intermediate weaning strategy, with brine shrimp feeding up to 40 dph, appears to be sufficient to convey important advantages in terms of growth and anatomy. Early weaning at 30 dph produced slow ‐ growing juveniles and a higher deformity ratio. Using the freeze dried preparations of the probionts did not affect the bacterial community structure of larvae and the probionts were not found to be established within the bacterial community of treated larvae. Poor quality egg may, however, partly explain the lack of effects as a result of treatment. The present study confirms the results of previous studies where live prey enrichment using a fish peptide hydrolyzate significantly improved larval survival and development. 

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Reports

Bioactive products in the production of halibut and cod larvae / Bioactive products in production of halibut and cod larvae

Published:

01/12/2008

Authors:

Jónína Þ. Jóhannsdóttir, Rannveig Björnsdóttir, Eydís Elva Þórarinsdóttir, Kristjana Hákonardóttir, Laufey Hrólfsdóttir

Supported by:

AVS, Matvælasetur HA

Bioactive products in the production of halibut and cod larvae / Bioactive products in production of halibut and cod larvae

The aim of the project was to find ways to improve the survival and quality of cod and halibut larvae and to use environmentally friendly methods. The aim was also to open up the possibility of utilizing saithe peptides that could increase the value of saithe. The results of a previous project in halibut farming were promising and indicated that it was most convenient to treat larvae with peptides through feed animals, in addition to which it was necessary to further investigate the concentration of treatment. In connection with the project, new facilities for animal husbandry have been developed and set up at Fiskey hf. for research into the different treatments of feed animals and thus contribute to increased stability in the production of halibut juveniles. Repeated experiments with bioactive substances in the cultivation of equidae have been carried out and they seemed to tolerate a certain concentration of the substances. The main results of experiments in the early stages of cod farming indicate that treatment with saithe peptides results in good growth, noticeably faster development of internal organs and a much lower incidence of larval defects. However, it is clear that the effects of different levels of treatment need to be further investigated. There is strong evidence that IgM and lysozyme are present in cod larvae soon after hatching or much earlier than previously claimed, and that treatment appeared to stimulate their production. Treatment with saithe peptides does not appear to affect the bacterial flora of larvae, but a specific species composition was detected in the gastrointestinal tract of larvae in pots where larval survival and quality were optimal. This gives evidence that a certain species composition of bacterial flora is favorable for cod larvae.

The main goal of this project was to increase viability and quality of cod and halibut larvae before and during the first feeding period by using bioactive products. The aim was also to increase the exploitation and value of pollock. The findings of previous projects in halibut culture were promising and indicated that treating live feed is a suitable method to carry bioactive products to the larval intestines during first feeding but the intensities of treatment needed to be further investigated. New facilities have been developed in relation to the project for research in the live feed culture at Fiskey Ltd. to promote increased stability in the production of halibut fingerlings. Repeated experiments have been conducted in the culture of rotifers and results indicate good tolerance towards treatment with bioactive products in certain intensities. The overall results of the project indicated that pollock peptides may promote increased growth and quality of cod larvae during first feeding. The results also indicate the presence of IgM and lysozyme early post hatching, but it has not been observed in cod larvae of this size before. Furthermore, results also indicate that hydrolysates from pollock can stimulate the production of these factors in cod larvae. Treatment using pollock peptides, did not affect the bacterial community structure of live feed or cod larvae, however a similar structure was observed in larvae from the most successful production units different from other tanks. The results therefore indicate a bacterial community structure that may be preferable to the cod larvae.

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