24-hour detection of undesirable microbes in food / 24-hour detection of undesirable microbes in food
The aim of the project was to develop and establish methods for rapid detection of undesirable bacteria in agricultural and marine products as well as other foods. With traditional methods as used today, results are obtained after 3 and up to 7 days, but with the methods developed in this project, it is possible to get results in a few hours or within 24 hours. The method is based on real-time PCR methodology and specific amplification of the genetic material of pathogenic bacteria and other undesirable bacteria. Diagnostic methods have been developed for major pathogens (Salmonella, Campylobacter, Listeria monocytogenes, Vibrio parahaemolyticus) in milk, meat and fish products as well as for specific spoilage bacteria in food. The results of the project will improve services to the food industry in Iceland by detecting unwanted microorganisms much earlier so that it is possible to intervene in production processes and thereby increase consumer safety in agricultural and fish products.
The aim of this project was to develop and set up new methods for rapid identification of undesirable bacteria in food and feed. With today's conventional and accredited culture methods results can be expected after 3 and up to 7 days. With the new methods to be taken in use and was developed in this project, the time of diagnostic procedure will decrease to few hours or to one working day. The detection methods are based on real ‐ time PCR technology and a specific amplification of genetic material of the undesired bacteria. Diagnostic methods for the most common pathogens (Salmonella, Campylobacter, Listeria monocytogenes, Vibrio parahaemolyticus) in meat‐, milk and fish products were developed through as well as quantitative assays for the main spoilage bacteria in fish. The results of the project will be used to improve the service for the Icelandic food industry on the domestic‐ and overseas markets by having rapid diagnostic methods for bacterial contamination at hand.