The short harvesting period of cultivated brown seaweed in Europe can make it difficult for cultivators to produce high quality seaweed biomass all year round. Hence there is a need for novel processing and preservation methods. Acid preservation is a well-known method to preserve food, where the aim is to reduce the pH below 4.5 to inhibit microbial growth. To evaluate the effectiveness of acid preservation, a shelf-life experiment was conducted with Saccharina latissima and Alaria esculenta. The biomass was either treated with lactic or citric acid and stored for approximately seven months. Physicochemical (including proximate composition, trace minerals, total phenolic content (TPC), texture and pH), microbial-, sensory attributes, and antioxidant (ORAC, DPPH) analyzes were performed on the preserved biomass during storage. The proximate composition, color, pH, and texture of the acid-preserved seaweed were relatively stable throughout the storage. However, a decrease was observed in TPC and antioxidant properties (assessed by DPPH) with the acid treatments. Acid preservation is, thus, a good method to stabilize the studied biomass for food and feed applications, but less applicable if intended for antioxidant purposes. However, the acid treated biomass might be suitable as an ingredient for a wide range of value-added products.