Genotyping Kits for Cod / Genotyping kits for Atlantic cod




Sigurlaug Skírnisdóttir, Inga Schulte, Sigurbjörg Hauksdóttir, Kristinn Ólafsson, Steinunn Magnúsdóttir, Klara Björg Jakobsdóttir, Christophe Pampoule, Guðmundur Ó. Hreggviðsson, Sigríður Hjörleifsdóttir

Supported by:

AVS Fisheries Research Fund


Sigurlaug Skírnisdóttir

Project Manager

Genotyping Kits for Cod / Genotyping kits for Atlantic cod

The aim of the project was to develop new coding sets for cod (Gadus morhua) based on repeated DNA short sequences (microsatellites). A total of 118 genetic markers were studied. Two ten genetic marker kits have been developed (CodPrint10a and CodPrint10b) and a patent application has been filed for these genetic markers. Almost 300 Icelandic samples belonging to 3 different sampling areas (N-Iceland, SW-Iceland (shallow waters) and SW-Iceland (deep) were analyzed with these 20 genetic markers, but for comparison the samples were also analyzed with nine well-known and widely used genetic markers. These three sample groups were better distinguished by CodPrint10a and CodPrint10b genetic markers than by previously known genetic markers, and the research shows that the new genetic markers are suitable for both stock studies and parental analyzes.

The goal of the project was to develop new genotyping kits for Atlantic cod (Gadus morhua) based on microsatellite markers. A total of 118 markers were analyzed. Two 10 microsatellite markers sets were developed (CodPrint10a and CodPrint10b) and they were used to analyze approximately 300 samples that were collected in the Northeast Iceland, Southwest inshore Iceland and Southwest offshore Iceland. As a comparison the samples were also analyzed with nine previously known markers. A comparison of the new microsatellite loci and the nine previously used, showed that the power of individual discrimination was much stronger with the new microsatellite loci. Indeed, the discrimination of the samples was clearer with much less overlap of the individuals. Together, these results suggest that the new microsatellite loci are powerful and suitable for both population genetic analysis and paternity analysis, due to their high polymorphism and resolution power.

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