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Aurélien Daussin

Specialist

aurelien@matis.is

Surface microbes are aerosolized into the atmosphere by wind and events such as dust storms and volcanic eruptions. Before they reach their deposition site, they experience stressful atmospheric conditions which preclude the successful dispersal of a large fraction of cells. In this study, our objectives were to assess and compare the atmospheric and lithospheric bacterial cultivable diversity of two geographically different Icelandic volcanic sites: the island Surtsey and the Fimmvörðuháls mountain, to predict the origin of the culturable microbes from these sites, and to select airborne candidates for further investigation. Using a combination of MALDI Biotyper analysis and partial 16S rRNA gene sequencing, a total of 1162 strains were identified, belonging to 72 species affiliated to 40 genera with potentially 26 new species. The most prevalent phyla identified were Proteobacteria and Actinobacteria. Statistical analysis showed significant differences between atmospheric and lithospheric microbial communities, with distinct communities in Surtsey's air. By combining the air mass back trajectories and the analysis of the closest representative species of our isolates, we concluded that 85% of our isolates came from the surrounding environments and only 15% from long distances. The taxonomic proportions of the isolates were reflected by the site's nature and location.

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Sixteen multiparous Holstein cows in four blocks of 4 × 4 Latin square over 4-week experimental periods were used to study the effects of seaweed (Saccharina latissima) supplement (with/without) and protein source (rapeseed meal (RSM)/wheat distiller's grain (WDG)) on milk mineral concentrations. Dietary treatments did not affect milk production and basic composition. Feeding seaweed slightly decreased milk Ca and Cu concentrations; whilst increased (by 3.3-fold) milk iodine (I) concentration, due to a higher dietary I supply. Substitution of WDG with RSM increased feed-to-milk transfer of Ca, Na, and Se and decreased that of Mg, P, Fe, and Mn; but only reduced milk Mn and I concentrations (the latter by 27 % as a potential result of increased glucosinolate intake). Seaweed supplement can improve milk I content when cows' I supply/availability is limited, but care should be taken to avoid excess milk I contents that may pose nutritional risks for young children.

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This study aimed to investigate the effects of two brown Icelandic seaweed samples (Ascophyllum nodosum and Fucus vesiculosus) on in vitro methane production, nutrient degradation, and microbiota composition. A total mixed ration (TMR) was incubated alone as control or together with each seaweed at two inclusion levels (2.5 and 5.0% on a dry matter basis) in a long-term rumen simulation technique (Rusitec) experiment. The incubation period lasted 14 days, with 7 days of adaptation and sampling. The methane concentration of total gas produced was decreased at the 5% inclusion level of A. nodosum and F. vesiculosus by 8.9 and 3.6%, respectively (P < 0.001). The total gas production was reduced by all seaweeds, with a greater reduction for the 5% seaweed inclusion level (P < 0.001). Feed nutrient degradation and the production of volatile fatty acids and ammonia in the effluent were also reduced, mostly with a bigger effect for the 5% inclusion level of both seaweeds, indicating a reduced overall fermentation (all P ≤ 0.001). Microbiota composition was analyzed by sequencing 16S rRNA amplicons from the rumen content of the donor cows, fermenter liquid and effluent at days 7 and 13, and feed residues at day 13. Relative abundances of the most abundant methanogens varied between the rumen fluid used for the start of incubation and the samples taken at day 7, as well as between days 7 and 13 in both fermenter liquid and effluent (P < 0.05). According to the differential abundance analysis with q2-ALEx2, in effluent and fermenter liquid samples, archaeal and bacterial amplicon sequence variants were separated into two groups (P < 0.05). One was more abundant in samples taken from the treatment without seaweed supplementation, while the other one prevailed in treatments supplemented with seaweed. This group also showed a dose-dependent response to seaweed inclusion, with a greater number of differentially abundant members between a 5% inclusion level and unsupplemented samples than between a 2.5% inclusion level and TMR. Although supplementation of both seaweeds at a 5% inclusion level decreased methane concentration in the total gas due to the high iodine content in the seaweeds tested, the application of practical feeding should be done with caution.

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Edible seaweeds with a relatively high total arsenic concentration have been a global concern. As the largest seaweed producer, China contributes about 60 % of the global seaweed production. The present study investigated 20 seaweed species collected from representative seaweed farming sites in the six provinces along the Chinese coastline, of which Saccharina japonica, Undaria pinnatifida, Neopyropia spp., Gracilaria spp., Sargassum fusiforme were listed as the most consumed seaweeds in the Food and Agriculture Organization of the United Nations (FAO). The inorganic arsenic (iAs) concentration in most of the seaweeds was below maximum limits (0.3 mg iAs/kg) as seaweed additives for infant food in the National Food Safety Standard of Pollutants in China (GB2762-2017, 2017), except for the species Sargassum, in which the iAs concentration significantly exceeded the limit and ranged from 15.1 to 83.7 mg/kg. Arsenic speciation in 4 cultivated seaweeds grown in both temperate and subtropical zones is reported for the first time. No significant differences in total As and iAs concentration were identified, except slightly higher total As concentration were found in Saccharina japonica growing in the temperate zone. The estimated daily intake (EDI) of toxic iAs via seaweed consumption was generally below the EFSA CONTAM Panel benchmark dose lower confidence limit (0.3 μg/kg bw/day) except for all Sargassum species where the EDI was significantly higher than 0.3 μg/kg bw/day. Moreover, the first-ever reported data on As speciation indicated very high iAs concentrations in Sargassum hemiphyllum and Sargassum henslowianum. To minimize the food chain iAs exposure, reducing both human intake of Sargassum spp. and the used of Sargassum spp. for animal feed is highly recommended.

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Thirty conventional and twenty-four organic dairy farms were divided into equal numbers within system groups: high-pasture, standard-pasture, and low-pasture groups. Milk samples were collected monthly for 12 consecutive months. Milk from high-pasture organic farms contained less fat and protein than standard- and low-pasture organic farms, but more lactose than low-pasture organic farms. Grazing, concentrate feed intake and the contribution of non-Holstein breeds were the key drivers for these changes. Milk Ca and P concentrations were lower in standard-pasture conventional farms than the other conventional groups. Milk from low-pasture organic farms contained less Ca than high- and standard-pasture organic farms, while high-pasture organic farms produced milk with the highest Sn concentration. Differences in mineral concentrations were driven by the contribution of non-Holstein breeds, feeding practices, and grazing activity; but due to their relatively low numerical differences between groups, the subsequent impact on consumers' dietary mineral intakes would be minor.

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The paper presents a synthesis of some of the interdisciplinary work from the ARCPATH project that focuses on the effects of climate change on Arctic social-ecological systems. It does so through the prism of whales and their recreational ecosystem services (ES). Whales present a group of species that are vulnerable to climate change and, at the same time, are central to the economies, cultures, and identities of many Arctic coastal communities. One such community is the town of Húsavík in Skj ́alfandi Bay, Iceland. The paper conducts an initial literature review to examine the effects of climate change on whales, globally, before using these findings and site-specific data from climate change modeling, whale observations from whale watching boats and whale watching trip records to investigate possible future impacts on whale watching in Skj ́alfandi Bay. The literature review identifies three categories of impacts on whales due to climate change, which concern changing distributions and migration, prey availability, and sea-ice and ocean temperature. Linear regression models identify statistically significant relationships between sea-surface temperatures (SST) and cetacean sightings for minke whales, blue whales and white-beaked-dolphins over the period 1995 to 2017. These species appear to have changed their usual feeding areas, and the results imply that further increases in SST are likely to further affect whale distributions. Future climate scenarios indicate that at least 2 ◦C of SST warming in Skj ́alfandi Bay up to 2050 might be inevitable regardless of the future emissions scenario, which implies almost certain change that would require adaptation. The reliance of the local tourism sector on whale watching makes Húsavík vulnerable to the effects of climate change on whales. The results of this interdisciplinary inquiry emphasize the interconnectedness of different components of social-ecological systems and calls for adaptation planning that would enhance the resilience of local communities to climate change and conservation measures that could enhance the protection of whales beyond the scope of the current whale sanctuary in Skjálfandi Bay.

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Pauline Bergsten

Ph.D. Student

paulineb@matis.is

The island of Surtsey was formed in 1963–1967 on the offshore Icelandic volcanic rift zone. It offers a unique opportunity to study the subsurface biosphere in newly formed oceanic crust and an associated hydrothermal-seawater system, whose maximum temperature is currently above 120 ° C at about 100m below surface. Here, we present new insights into the diversity, distribution, and abundance of microorganisms in the subsurface of the island, 50years after its creation. Samples, including basaltic tuff drill cores and associated fluids acquired at successive depths as well as surface fumes from fumaroles, were collected during expedition 5059 of the International Continental Scientific Drilling Program specifically designed to collect microbiological samples. Results of this microbial survey are investigated with 16S rRNA gene amplicon sequencing and scanning electron microscopy. To distinguish endemic microbial taxa of subsurface rocks from potential contaminants present in the drilling fluid, we use both methodological and computational strategies. Our 16S rRNA gene analysis results expose diverse and distinct microbial communities in the drill cores and the borehole fluid samples, which harbor thermophiles in high abundance. Whereas some taxonomic lineages detected across these habitats remain uncharacterized (eg, Acetothermiia, Ammonifexales), our results highlight potential residents of the subsurface that could be identified at lower taxonomic rank such as Thermaerobacter, BRH-c8a (Desulfallas-Sporotomaculum), Thioalkalimicrobium, and Sulfurospirillum. Microscopy images reveal possible biotic structures attached to the basaltic substrate. Finally, microbial colonization of the newly formed basaltic crust and the metabolic potential are discussed on the basis of the data.

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Pauline Bergsten

Ph.D. Student

paulineb@matis.is

The oceanic crust is the world's largest and least explored biosphere on Earth. The basaltic subsurface of Surtsey island in Iceland represents an analogue of the warm and newly formed oceanic crust and offers a great opportunity for discovering novel microorganisms. In this study, we collected borehole fluids, drill cores, and fumarole samples to evaluate the culturable bacterial diversity from the subsurface of the island. Enrichment cultures were performed using different conditions, media and temperatures. A total of 195 bacterial isolates were successfully cultivated, purified, and identified based on MALDI-TOF MS analysis and by 16S rRNA gene sequencing. Six different clades belonging to Firmicutes (40%), Gammaproteobacteria (28.7%), Actinobacteriota (22%), Bacteroidota (4.1%), Alphaproteobacteria (3%), and Deinococcota (2%) were identified. Bacillus (13.3%) was the major genus, followed by Geobacillus (12.33%), Enterobacter (9.23%), Pseudomonas (6.15%), and Halomonas (5.64%). More than 13% of the cultured strains potentially represent novel species based on partial 16S rRNA gene sequences. Phylogenetic analyzes revealed that the isolated strains were closely related to species previously detected in soil, seawater, and hydrothermal active sites. The 16S rRNA gene sequences of the strains were aligned against Amplicon Sequence Variants (ASVs) from the previously published 16S rRNA gene amplicon sequence datasets obtained from the same samples. Compared with the culture-independent community composition, only 5 out of 49 phyla were cultivated. However, those five phyla accounted for more than 80% of the ASVs. Only 121 out of a total of 5642 distinct ASVs were culturable (≥98,65% sequence similarity), representing less than 2,15% of the ASVs detected in the amplicon dataset. Here, we support that the subsurface of Surtsey volcano hosts diverse and active microbial communities and that both culture-dependent and -independent methods are essential to improving our insight into such an extreme and complex volcanic environment.

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Pauline Bergsten

Ph.D. Student

paulineb@matis.is

Novel thermophilic heterotrophic bacteria were isolated from the subsurface of the volcanic island Surtsey off the south coast of Iceland. The strains were isolated from tephra core and borehole fluid samples collected below 70 m depth. The Gram-negative bacteria were rod-shaped (0.3–0.4 µm wide, 1.5–7 µm long), aerobic, non-sporulating and non-motile. Optimal growth was observed at 70 °C, at pH 7–7.5 and with 1% NaCl. Phylogenetic analysis identified the strains as members of the genus Rhodothermus. The type strain, ISCAR-7401 T, was genetically distinct from its closest relatives Rhodothermus marinus DSM 4252 T and Rhodothermus profundi PRI 2902 T based on 16S rRNA gene sequence similarity (95.81 and 96.01%, respectively), genomic average nucleotide identity (73.73 and 72.61%, respectively) and digital DNA–DNA hybridization (17.6 and 16.9%, respectively). The major fatty acids of ISCAR-7401 T were iso-C 17:0, anteiso-C 15:0, anteiso-C 17:0 and iso-C 15:0 (>10 %). The major isoprenoid quinone was MK-7 while phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminophospholipid and a phospholipid were the predominant polar lipid components. Based on comparative chemotaxonomic, genomic and phylogenetic analyses, we propose that the isolated strain represents a novel species of the genus Rhodothermus with the name Rhodothermus bifroesti sp. Nov. The type strain is ISCAR-7401 T (=DSM 112103 T =CIP 111906 T).

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