Peer-reviewed articles

Chloroplast markers for the Malvaceae and the plastome of Henderson's checkermallow (Sidalcea hendersonii S.Wats.), a rare plant from the Pacific Northwest. 

Contact

Sæmundur Sveinsson

Research Group Leader

saemundurs@matis.is

Objective

Sidalcea is a genus of flowering plants restricted to the west coast of North America, commonly known as checkermallows. Remarkably, of the ~ 30 recognized species, 16 are of conservation concern (vulnerable, imperiled or critically imperiled). To facilitate biological studies in this genus, and in the wider Malvaceae, we have sequenced the whole plastid genome of Sidalcea hendersonii. This will allow us both to check those regions already developed as general Malvaceae markers in a previous study, and to search for new regions.

Results

By comparing the Sidalcea genome to that of Althaea, we have identified a hypervariable approximately 1 kb region in the short single copy region. This region shows promise for examining phylogeographic patterns, hybridization and haplotype diversity. Remarkably, considering the conservation of plastome architecture between Sidalcea and Althaea, the former has a 237 bp deletion in the otherwise highly conserved inverted repeat region. Newly designed primers provide a PCR assay to determine the presence of this indel across the Malvaceae. Screening of previously designed chloroplast microsatellite markers indicates two markers with variation within S. hendersonii that would be useful in future population conservation genetics.

Reports

Geothermal Habitats and Adaptations of Thermophilic Microbes

Published:

10/11/2023

Authors:

Björn Þór Aðalsteinsson, Guðmundur Óli Hreggviðsson

Contact

Björn Þór Aðalsteinsson

Project Manager

bjornth@matis.is

Chapter in Thermophilic Anaerobes (pp.3-19):

In this chapter, the main habitats of thermophiles, their discovery, and ecology are discussed. The focus of the discussion is on natural habitats associated with geothermal activity, their geological origin, and characteristics of different geothermal surface manifestations, including mud pools, solfatara fields, alkaline hot springs, and warm springs. The ecological discussion is primarily focused on strategies that thermophiles utilize to obtain energy.

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Reports

Valorisation of side streams

Published:

29/12/2023

Authors:

Eva Margrét Jónudóttir, Matís; Ólafur Reykdal, Matís; Helga Gunnlaugsdóttir, Orkídea; Sophie Jensen, Matís; Léhna Labat, Matís; Þóra Valsdóttir, Matís; Guðrún Birna Brynjarsdóttir, Farmers Association of Iceland; Valur Klemensson, Farmers Association of Iceland and Rósa Jónsdóttir, Matís

Supported by:

Food Foundation / Icelandic Food Innovation Fund

Contact

Eva Margrét Jónudóttir

Project Manager

evamargret@matis.is

The main goal of the project "Value addition of side products from horticulture" was to find ways to use side products from horticulture production for increased value creation and also to reduce waste in vegetable production. Different by-products were examined, including what falls from the defoliation of tomato and cucumber plants, leaves from outdoor vegetables such as cauliflower and broccoli, as well as leaves and stems from rose cultivation. The basis for improved utilization of secondary products and excess quantities of potatoes and carrots through biomass processing and product development was also examined. It was examined whether the consumption of by-products was safe with appropriate risk analysis and chemical measurements, and the main information on possible negative health effects was compiled.

Information on the chemical content of different by-products is now available. A significant number of samples reached the limit for several additives, viz. 15% of the nutritional reference value (NV), and thus there is a possibility of labeling the relevant supplement on the packaging of by-products if they are sold directly as food. Biomass processing of second class potatoes and beets was tested and the total amount of polyphenols as well as antioxidant activity was investigated. The total amount of polyphenols and antioxidant activity were studied in leaves and branches of cauliflower, broccoli, tomato, cucumber and roses. The total amount of polyphenols was high in extracts of rose petals and branches as well as antioxidant activity and "anti-aging" activity. Subsequently, it was decided to test its use as an ingredient in skin care products. The processing characteristics of yellow beets were examined in terms of shelf life and sensory aspects. Two different recipes of spice mixes were developed that included both side products from cauliflower and broccoli cultivation. Products from biomass processing such as starch and fiber were tested in the recipes of the spice mixture with reasonable results. There can be various shortcomings in the use of horticultural by-products in food, and several things must be considered. The main hazards associated with the consumption of vegetables and their by-products can be classified into microbial contamination, unwanted substances and foreign bodies.

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Reports

Atlantic salmon digestibility and faeces stability trial

Published:

29/12/2023

Authors:

David Sutter, Wolfgang Koppe, Sven-Ole Meiske and Georges Lamborelle

Supported by:

Mowi Feed AS

Contact

Georges Lamborelle

Station manager of Matís Aquaculture Research Station

georges@matis.is

This report is closed.

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Reports

Microorganisms for aquaculture sludge enrichment   

Published:

21/12/2023

Authors:

Anna Berg Samúelsdóttir, Matís, Alexandra Leeper, Sjávarklasinn, Clara Jégousse, Sjávarklasinn, Ólafur H. Friðjónsson, Matís, Elísabet Eik Guðmundsdóttir, Matís, Hörður Guðmundsson, Matís and Birgir Örn Smárason, Matís

Supported by:

Hringrásarsjóður

Contact

Anna Berg Samúelsdóttir

Project Manager

annab@matis.is

The main goal of the project "Microbes for the enrichment of aquaculture sludge" was to develop a method for treating side streams from aquaculture (sludge) with microorganisms so that the sludge can be used as fertilizer for agriculture.  

Considering the rapid growth of aquaculture in Iceland, it is crucial for the sustainability of the industry to find solutions for side currents and thus strengthen the circular economy. The implementation of solutions that promote the use of side streams, and promote circulation, are in line with the United Nations' goals for sustainable development. 

The legal framework for the use of fish farm sludge as fertilizer is both extensive and in parts quite complex, i.e. what is allowed and who grants permission. As an example of the requirements for using sludge as fertilizer, sludge must be applied to the pasture before December 1st if the area is to be used for grazing, animals can then be grazed on the area 5 months later or on April 1st at the earliest.  

In the project, work was done to enrich nitrate in the sludge with microorganisms to increase the possibility of using the sludge as a fertilizer. An enrichment culture was established with the aim of enriching ammonia-oxidizing bacteria in the sludge. A chemical analysis of the sludge was also carried out to assess its nutrient content. The results of chemical measurements indicate that sludge can be ideal as a supplement or additive to, for example, biodegradable livestock manure. It is important to continue with a project that contributes to increasing the value of by-products such as sludge in order to keep nutrients within the circular economy. The use of sludge as fertilizer is beneficial for both aquaculture companies and Icelandic agriculture.  
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The primary objective of the project "Microorganisms for aquaculture sludge enrichment" was to develop a method for treating side streams from aquaculture (sludge) using microorganisms, thereby rendering the sludge suitable for use as agricultural fertilizer. 

Given the rapid expansion of aquaculture in Iceland, finding solutions for side streams is imperative to sustain the industry and enhance circular economy practices. Implementing solutions that encourage side stream utilization aligns with the United Nations' sustainable development goals. 

The legal landscape for utilizing fish farm sludge as fertilizer is extensive and, in certain aspects, complex, delineating what is permissible and who grants permission. For instance, applying sludge to pasture for grazing requires adherence to specific timelines, such as application before December 1st, with grazing permitted no earlier than 5 months later or on April 1st. 

The project focused on enriching the sludge's nitrogen content with microorganisms. An enrichment culture was established to promote ammonia-oxidizing bacteria in the sludge, increasing its potential as a fertilizer. Chemical analysis of the sludge was conducted to evaluate its nutrient content. The results indicate that the sludge can serve as an ideal supplement or additive, for instance, with biodegradable livestock manure. Continuing projects that enhance the value of like sludge is crucial for maintaining nutrient cycles within the circular economy. The use of sludge as fertilizer is mutually beneficial for both aquaculture companies and Icelandic agriculture. 

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Reports

Matís' report to NÍ 2023: Sampling and shipments 01.11.2022 – 31.10.2023

Published:

21/12/2023

Authors:

Elísabet Eik Guðmundsdóttir

Contact

Elísabet Eik Guðmundsdóttir

Project Manager

elisabet@matis.is

The sampling period covered by this report is from November 1, 2022 – October 31, 2023. A total of 328 samples were taken during the period, of which 306 were for research within the EUROPLANET collaboration, 21 for the mapping of marine microbes in Iceland and one for the CAZyme-X project. 

Reports

The effect of subcategories in the EUROP fat classification of lamb carcasses on meat quality

Published:

20/12/2023

Authors:

Guðjón Þorkelsson, Aðalheiður Ólafsdóttir, Eva Margrét Jónudóttir and Óli Þór Hilmarsson

Supported by:

Sheep farming development capital

Contact

Guðjón Þorkelsson

Strategic Scientist

gudjon.thorkelsson@matis.is

The aim of this study was to investigate the effect of a 15-point fat evaluation of lamb carcasses on fat in the back muscle and its taste and texture. A small relationship was found between fat categories in the European assessment and fat in spinal muscles and fat explosion by visual assessment. Fat groups had rather little effect on taste and texture. Spinal muscles in fat category 3+ were significantly softer, more tender and juicier than from other categories. Spinal muscles from fat group 2- were the least tender and the least juicy of all the groups in the experiment. It is interesting that out of about 14.5% of carcasses of lambs slaughtered in the second slaughter at the experimental farm LBHÍ went to the European subcategory 3+. There are possibly opportunities to revise the fat classification and process this meat and sell it as a special quality product. Little fat was measured in spinal muscles or in the range 1.1% – 3.6% and on average 1.92%. The reason may be the young age at slaughter, the Icelandic sheep breed and possibly breeding for a lot of muscle and lean meat.
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The effects of subclasses of EU fat classification of lamb carcasses on chemical fat, visual marbling scores and eating quality of loin muscle (m. longissimus dorsi) were studied. Correlation coefficients between fat classes, chemical fat content and marbling scores were low. The only effects of fat classes on eating quality were that class 2- had the least tender and juicy loin muscles and class 3+ loin muscles were the softest, most tender and juicy. 14,5% of the 317 carcasses of the lambs slaughtered were classified as 3+ according to the EU guidelines. Maybe there is an opportunity to review the rules in Iceland and separate the carcasses with the higher quality meat and market them as such. Intramuscular fat was low or from 1.1% – 3.9% and averaging 1.92%. The reasons may be young age at slaughter, the Icelandic sheep breed and maybe breeding for leaner meat.

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Reports

Study on the effects of raw materials on smoltification performance of Atlantic Salmon

Published:

19/12/2023

Authors:

David Sutter, Sven-Ole Meiske, Wolfgang Koppe & Georges Lamborelle

Supported by:

Darling

Contact

Georges Lamborelle

Station manager of Matís Aquaculture Research Station

georges@matis.is

This report is closed.

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Reports

Ratio of meat, fat and bones in lamb meat – Chemical content of lamb meat and by-products 

Published:

15/12/2023

Authors:

Óli Þór Hilmarsson, Ólafur Reykdal, Guðjón Þorkelsson, Helgi Briem and Hafliði Halldórsson

Supported by:

Icelandic Food Innovation Fund (is. Matvælasjóður)

Contact

Ólafur Reykdal

Project Manager

olafur.reykdal@matis.is

In the project, an assessment was made of the ratio of meat, fat and bones in lamb meat. Carcasses from the meat evaluation categories O-2, R-2, R-3, U-2, U-3, U-3+, and E-3 were selected for the evaluation, nine carcasses from each evaluation category, a total of 63 carcasses. Carcasses from these categories cover the 92% production based on the division into meat food categories in 2021. Carcasses were selected on three different slaughter days, in two slaughterhouses, in the north and the south, in such a way that the head of the meat evaluation department at the Food Agency, selected all carcasses and confirmed that each a carcass would be a traditional carcass in its assessment category and not at the edge of the category. The day after slaughter, the carcasses were split in half. One half was divided into thighs, forequarters, loins and loins according to the traditional division, while the other half was divided into three weight categories, lightweight under 14.5 kg, medium 14.5 - 16.8 kg and heavy 16.9 - 19.0 kg. The halves were then divided in different ways, with parts going into the various products. Precision deboning was applied to both halves of the lamb carcasses to find the division of the various cuts and products into meat, fat, bone and sinew. Atrophy was also found due to loss of ossification. 

Meat utilization (meat ratio) for the lamb carcasses as a whole was 59.0 (50.7-67.3)%, fat ratio was 16.2 (9.7-28.0)%, bone ratio was 17.7 (13.4- 22.1)% and the tendon ratio was 6.3 (4.4-8.1)%. Atrophy during precision boning was 1.1 (0.0-2.5)%. The average meat utilization was highest in the evaluation category U-2, except for the front part, where the utilization was somewhat higher in E-3. Within the flesh filling categories U and R, it was clearly seen how the percentage of fat changes according to the definitions of fat categories. 

The proportion of meat, fat and bones in different quality categories confirms that the meat assessment is realistic and in accordance with the definitions behind the assessment. 

The proportions of meat, fat, bones, tendons and shrinkage were found for 30 lamb products from selected weight classes. High meat percentage was obtained for thigh without rump, hip bone and tail bone from heavy carcasses (74%) and thigh with shank without hip from both light and medium carcasses (69%). 

Measurements were made of nutrients in lamb pieces and lamb products. Heavy metal measurements were made on lamb pieces. These results will be useful for packaging labeling and for providing information to consumers and retailers. The lamb meat was so rich in vitamin B12, vitamin folate, potassium and zinc that it is permissible to label these substances as part of the meat's nutrition label on the packaging. The heavy metals mercury, cadmium, lead and arsenic were not measurable in the meat, i.e. were below the limits that could be safely measured. This limit is very low and therefore the possible concentration of heavy metals is extremely low.  

Sampling of lamb offal and other side products took place in three slaughterhouses, at SS in Selfoss, KS in Sauðárkrók and Norðlenska in Húsavík. Samples were obtained from liver, kidney, heart, lung, testicle, esophagus, pancreas, spleen, and blood. Chemical measurements were made on selected nutrients and heavy metals. The lamb offal and by-products are rich in iron and selenium, but these substances are important nutrients. All samples reach significant levels of selenium. In the case of significant quantities, labeling of food packaging is permitted according to the labeling regulation. Most of the samples reached significant levels of iron. The heavy metal cadmium was detectable in liver and kidney but not in other samples. Mercury, lead and arsenic were not measurable in the samples, although with the exception that mercury in the kidneys was measurable. 

Chemical measurement results call for attention and improvements in labeling and information. 
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Muscle, fat and bone ratios of Icelandic lamb meat were studied. Carcasses from the EUROP grades: O-2, R-2, R-3, U-2, U-3, U-3+, and E-3 were selected, nine carcasses from each grade, a total of 63 carcasses. Carcasses from these grades represent 92% of the lamb meat production in Iceland as reported for 2021. Carcasses were selected during three slaughtering days, in two slaughterhouses in north and south Iceland. The grade classifications of carcasses were confirmed by a specialist from the Icelandic Food and Veterinary Authority. 

The carcasses were divided into halves the day after slaughtering. One half was divided into traditional leg, forequarter, saddle, and flank. The other half was used for the study of various cuts, where each product was made from one of three selected carcass weight ranges: light carcasses below 14.5 kg, medium carcasses 14.5-16.8 kg and heavy 16.9-19.0 kg. Boning was carried out on all products and dissection yields were reported (meat, fat, bones, tendons). Wastage due to cutting, and boning was reported. 

Tissue ratio for whole lamb carcasses were on average 59% meat, 16% fat, 18% bones, and 6% tendons. Wastage during thawing and cutting was 1.1%. The meat yields were highest for grade U-2, except for forequarter which had a bit higher meat yield for grade E-3. For grades U and R, it was clear that fat yields were related to the definitions of fat thickness for the grade subgroups 2, 3 and 3+. 

Dissection yields were reported for meat, fat, bones, and tendons in 30 meat products made from carcasses of different weights. Highest meat yields were for leg products (74% and 69%). 

Selected nutrients were analyzed in legs, forequarters, saddles, flanks, and several other cuts. The results will be used for labeling and dissemination. Lamb meat was rich in vitamin B12, folate, potassium, and zinc. These nutrients can be used for nutrition declarations of the meat. The heavy metals mercury, cadmium, lead and arsenic were not detected (were below the detection limits) in lamb meat. The detection limits were very low. 

Sampling of lamb side-products were carried out in three slaughterhouses, at Selfoss, Sauðárkrókur and Húsavík. The following side-products were sampled: Liver, kidneys, heart, lungs, testis, esophagus, pancreas, spleen, and blood. Selected nutrients and heavy metals were analyzed. The side-products were generally rich in selenium and iron which can be used for nutrition declarations in most cases. The heavy metal cadmium was reported for liver, and kidneys, cadmium was however not detected in other side-products. Mercury was only detected in the kidneys. Lead and arsenic were not detected in the side-products. 

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Reports

Product development of ready-to-heat vegetarian meals containing Torula

Published:

14/12/2023

Authors:

Kolbrún Sveinsdóttir, Aðalheiður Ólafsdóttir and Birgir Örn Smárason

Supported by:

Grant Agreement: 862704, Horizon 2020, EU Research and Innovation program

Contact

Kolbrún Sveinsdóttir

Project Manager

kolbrun.sveinsdottir@matis.is

Availability of high-quality, sustainably produced protein is becoming increasingly limited, but at the same time, global demand for protein has never been higher. Increased emphasis has been placed on more sustainable consumption habits and lifestyles in Western societies with the aim of reducing negative environmental impacts. To meet the increased need for protein, consumers need to reduce their consumption of animal protein and increase their consumption of other proteins. Such proteins are not only traditional plant proteins but also neoproteins such as monocytes which are relatively new on the European food market.

Within the European project NextGenProteins, the food producer Grímur Kokkur, in collaboration with Matís, developed vegetarian dishes that contained the protein source Torula. The focus was mainly on prepared vegetable dishes that contain neoproteins in such amounts that the dish can be labeled as a source of protein (at least 12% calories (kcal) from protein out of total calories).

This report describes trials on the development of breaded vegetable buns containing Torula and date filling and breaded risotto buns containing Torula. Sensory properties, nutrients and consumer experience of the cups were evaluated. Sensory evaluations performed by trained sensory judges did not indicate any defects in sensory quality and consumer feedback was generally very positive. Furthermore, both products contained more than 12% kcal from proteins. From these results, it can be concluded that the product development of prepared vegetable dishes containing Torula has been successful.
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Access to high quality, sustainably produced proteins is becoming increasingly restricted due to a growing world population, increased pressure on natural resources and climate change, while at the same time the global protein demand has never been higher. There has been increased emphasis towards more sustainable consumption habits and lifestyle in western societies with the aim to reduce negative environmental effects. To meet the increased need for protein, consumers need to reduce their consumption of animal protein and increase their consumption of alternative proteins. Alternative proteins are not only traditional plant proteins but also from novel sources such as single cells which are relatively new to the food market in Europe. 

Within the European project, NextGenProteins, the food producer Grímur Kokkur developed a series of vegetarian meals containing the alternative protein source Torula in collaboration with Matís. The focus was mainly placed on vegetarian ready meals containing the alternative protein ingredient in a ratio which enables the producer to claim the ready meals are a source of protein (at least 12% kcal from protein of total kcal).

This report summarizes and describes trials of the development of breaded vegetarian cakes containing Torula and date filling and breaded risotto cakes containing Torula, with regard to sensory characteristics, nutrients and consumer liking. Sensory evaluation by trained sensory panelists indicated no defects regarding sensory quality, and both products were very well perceived by consumers. Furthermore, the products both contain more than 12% kcal from proteins. From these results it can be concluded that the product development of the oven-ready vegetarian meals with Torula was successful.

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