Reports

Microbial diversity in hot springs in Vonarskarð / Microbial diversity in hot springs in Vonarskarð

Published:

01/03/2009

Authors:

Sólveig K. Pétursdóttir, Snædís H. Björnsdóttir, Sólveig Ólafsdóttir, Guðmundur Óli Hreggviðsson

Supported by:

Orkustofnun due to the Master Plan

Contact

Guðmundur Óli Hreggviðsson

Strategic Scientist

gudmundo@matis.is

Microbial diversity in hot springs in Vonarskarð / Microbial diversity in hot springs in Vonarskarð

Samples were taken in the geothermal area east of Eggja in Vonarskarð. A total of 32 samples were taken from different hot springs, streams and soils at different temperatures and acidity levels. Elements were measured in several water samples. The species composition of bacteria and ancient bacteria was determined by molecular biological methods. A total of 1052 16S rRNA gene sequences of bacteria were detected, spreading across 23 factions. More than 50 new bacterial species were found in the samples, of which 11 are probably representatives of new genera. The genome of ancient bacteria was 155 in number. Ten new species of ancient bacteria were found, including five representatives of new genera. The ecosystem of the geothermal area as a whole must therefore be considered very special. The most common were the primitive species of the array Aquificae. Primitive and non-primitive Proteobacteria species were found in significant quantities and were both known and new species. Typical species of bluish-green bacteria and Chloroflexi were found in the samples. Firmicutes, Bacteroidetes and Chlorobi species were found mainly in samples taken at lower temperatures. Ancient bacteria found in the samples were spread over two largest groups of ancient bacteria, i.e. Crenarcheota and Euryarchaeota. The biological diversity of bacteria and archaeal bacteria in the samples was most often in the range Nt / Nmax = 1.0 -3.0, which is typical of peripheral ecosystems. In some cases it was higher, especially in samples where the temperature was relatively low and therefore more viable for more species. Seven bacterial species were cultured from the samples, including one new species of the genus Sediminibacter from the Bacteroidetes class. Several Thermus species were bred, including T.islandicus which is native to Iceland. The proteobacteria Thermomonas hydrothermalis and Tepidimonas ignava were also grown from several samples and the thermophilic Firmicutes species Anoxybacillus kualawohkensis.

Samples were taken from the geothermal area east of Eggja in Vonarskarð. A total of 32 samples were collected from different sites at various temperature and pH values. The concentration of 72 elements were estimated in water samples. Species composition of Bacteria and Archaea was estimated using molecular methods. A total of 1052 16S rRNA gene sequences belonging to 23 bacterial phyla were detected. Roughly 50 novel bacterial species were found of which 11 represent new genera. Ten novel archaeal species were found, five of which represent new genera. Species belonging to the autotrophic phylum of Aquificae dominated many samples. Species of different subphyla of Proteobacteria were also represented in high ratios in the samples, both described and novel species. Common species of Cyanobacteria and Chloroflexi were also detected. Species of the Firmicutes, Bacteroidetes and Chlorobi phyla were common in samples taken at lower temperatures. Archaeal species in the samples belonged to both Crenarchaeota and Euryarchaeota. The calculated biodiversity index for bacteria and archaea in the samples was 1,0-3,0 which is in concordance with values obtained for extreme ecosystems. It was higher in a few samples which were taken at lower temperatures and thus represent habitats acceptable for more diverse organisms. Seven bacterial species were isolated from the samples. One of these represents a novel species of the genus Sediminibacter within the phylum of Bacteroidetes. Several Thermus species were cultivated, ie T.islandicus which has so far only been found in Iceland. The Proteobacteria species Themomonas hydrothermalis and Tepidimonas ignava were also isolated as well as a thermophilic Firmicutes species, Anoxybacillus kualawohkensis.

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Reports

Production of wheeled animals for cod farming / Production of quality rotators for breeding cod fry

Published:

01/03/2009

Authors:

Jónína Þ Jóhannsdóttir, Agnar Steinarsson, Rannveig Björnsdóttir

Supported by:

Nordic-Atlantic co-operation (NORA)

Production of wheeled animals for cod farming / Production of quality rotators for breeding cod fry

There is great interest in finding ways to control farming conditions at all stages of aquaculture. The control of microbial flora in the environment and the gastrointestinal tract of larvae is, among other things, thought to be able to reduce losses that occur in the early stages of marine fish farming. A large number of bacteria usually accompanies feed animals in cod farming, but the use of recycling systems in the breeding of equidae has, among other things, the advantage that a smaller number of bacteria gain a foothold in the systems compared to batch farming. The use of bacterial flora for bacterial flora control has increased significantly in recent years and has in some cases contributed to increased growth and quality of larvae. In addition to strengthening co-operation between cod juvenile producers in the Nordic countries, the aim of the project is to develop methods that increase stability and efficiency in the production of feed animals. It is based on a recycling system designed by Sintef in Norway and this section investigates the effects and presence of selected beneficial bacteria in the system. The main results of treatment with two selected bacterial strains showed that the wheeled animals tolerated the treatment well and an increased crop of animals was obtained when treated with the bacteria in lyophilized form. There was a significant decrease in the number of bacteria in the purgatory during treatment with liquid bacterial culture and the number of bacteria in the purgatory did not reach the initial number during the experiment, but there was some increase in the number of lactic acid bacteria. Although the amount of lactic acid bacteria in equidae only increased after treatment, the bacterial strains did not gain a foothold in the system or lead to changes in the composition of the bacterial flora when treated at this concentration. The project is funded by Nordisk Atlantsamarbejde (NORA) and worked in collaboration with Matís, the Marine Research Institute, SINTEF, Fiskaaling, IceCod and Stofnfisk as well as Nordland Marin Yngel.

There is an increasing interest in controlling environmental parameters during the first production stages of aquaculture and controlling bacterial numbers is among various environmental parameters that are believed to promote increased survival of larvae. Elevated bacterial numbers are introduced into the system through the live feed, but numbers of bacteria have been found to be greatly reduced by the use of recirculation culturing system as compared with batch culturing systems. Furthermore, the use of potentially probiotic bacteria in aquaculture has increased over the past years and has in some cases contributed to increased growth and quality of marine larvae. In addition to promoting collaboration between cod producers within the Nordic countries, the main goal of the current project is to develop methods for stable and advantageous production of live feed animals (rotifers). The project is based on a recirculation culturing system engineered by SINTEF and the present part of the project deals with the effect of treatment and persistence of selected probiotic bacterial strains in the system. The overall results indicate that the rotifer cultures were not negatively affected by the bacterial treatment and treatment using freeze-dried preparations of the two probiotic strains even resulted in improved harvesting of the live feed. A drop in bacterial numbers within the bio-filter unit was, however, observed following the addition of liquid bacterial cultures, indicating negative effects of the bacteria on the bacterial community of the bio-filter unit. An increase in the numbers of lactic acid bacteria was observed in the rotifer cultures following treatment, but the probiotic bacterial strains were neither found to become established as a part of nor affect the dominating bacterial community of the system using the concentrations applied. The project was supported by the Nordisk Atlantsamarbejde (NORA) and is a collaboration between Matís, Hafrannsóknastofnun, SINTEF, Fiskaaling, IceCod, Stofnfiskur and Nordland Marin Yngel.

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Reports

Air-dried lamb. Forathugun / Air dried products from lamb

Published:

01/03/2009

Authors:

Þóra Valsdóttir, Óli Þór Hilmarsson, Guðjón Þorkelsson

Supported by:

Technology Development Fund

Contact

Þóra Valsdóttir

Project Manager

thora.valsdottir@matis.is

Air-dried lamb. Forathugun / Air dried products from lamb

The aim of the project is to prepare a collaborative project between parties in Iceland, the Faroe Islands and Norway on the development of air-dried lamb products in connection with the establishment of small start-up companies and food tourism.

The report includes a summary and analysis of the state of air drying in Iceland and a survey of market and commercial criteria. The analysis is divided into:

(1) a survey of the status of air-dried lamb in Iceland

(2) the impact of production methods on efficacy, quality and safety: a summary of technical and safety considerations; and

(3) a summary of the criteria for marking the origin and protection of certain products.

Finally, the selection of partners and the formulation of projects related to air drying of lamb are explained.

The aim of the project is to prepare a cooperative project between parties in Iceland, Faroe Islands and Norway on development of new air-dried products from lamb. The product development will be done in relation with establishment of small companies and food tourism.

The report is a summation and analysis on the situation of air drying in Iceland and exploration of market and business-related issues. The analysis is divided into:

(1) exploration on the situation of air dried lamb in Iceland

(2) influence of production methods on curing, quality and safety

(3) summation of criterion for origin-based labeling and protection of specific products.

Finally, established cooperation and creation of projects linked to air dried lamb is listed.

Report closed until 01.04.2012 / Report closed until 01.04.2012

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News

Summary of previous research on capelin roe - Matís report

A new report has been published by Matís. The report is a summary of previous research on capelin roe.

In recent years and decades, various measurements and research on capelin roe in Iceland have been carried out at Matís ohf / Rannsóknastofnun fiskiðnaðarins. This is primarily a study of microorganisms, egg filling and water content. The report will cover microbial research carried out during the 1984 season, microbiological measurements in the period 2000-2008 and measurements of water content and egg filling 1984-2008.

The information that can be found there is exciting and relevant these days, as people are talking about the importance of capelin for the country's economy.

The full report can be found here.

For further information, please contact Margeir Gissurarson, margeir.gissurarson@matis.is.

News

Hrönn Ólína Jörundsdóttir is defending her doctoral dissertation in environmental chemistry

Matís' employee, Hrönn Ólína Jörundsdóttir, defended her doctoral dissertation "Temporal and spatial trends of organohalogens in guillemot (Uria algae) in North Western Europe" on February 6, 2009 at Umildfild Stockholm University.

The dissertation deals extensively with persistent organic pollutants in the Nordic countries, such as PCBs and the insecticide DDT, as well as their metabolites, which were mainly measured in long-range eggs. The substances were measured in eggs from Iceland, the Faroe Islands, Norway and Sweden to obtain a geographical comparison.

The results show that the North Atlantic ecosystem is less polluted than the Baltic ecosystem, but various pollutants were found to be in similar concentrations in these areas and it is necessary to investigate further why this is the case. Fluorinated alkanes, which come from outdoor clothing, have recently been found in significant amounts in nature, measured in long-tailed eggs from Iceland, the Faroe Islands, Norway and Sweden and were in some cases in higher concentrations in eggs from the North Atlantic than in the Baltic Sea. Brominated fire retardants, which are used in electronics, among other things, were measurable everywhere and it seems that it is possible to identify the different origins of the substances that reach the North Atlantic, on the one hand from North America and on the other from Europe.

A comparison was also made of the amount of pollutants in seven Icelandic bird species, i.e. heron, eider, osprey, elephant, herring gull, black-backed gull and skimmer. The foam was found to have a significant high concentration of pollutants, including PCB compounds and the insecticide DDT, and it is important to study the health condition of the foam.

It is clear that part of the pollution measured in the Icelandic ecosystem travels through ocean and air currents to Iceland, but on the other hand a significant part is due to Icelanders' use of goods that contain various pollutants.

Supervisors were dr. Åke Bergman Professor of Environmental Chemistry at Stockholm University, dr. Professor Anders Bignert, Swedish Museum of Natural History and dr. Mats Olsson Professor Emeritus. The opponent was Dr. Derek Muir, Environment Canada.

The examination committee consisted of dr. Kristín Ólafsdóttir, Associate Professor at the University of Iceland, dr. Björn Brunström, professor at Uppsala University and dr. Conny Östman, Associate Professor at Stockholm University.

For further information, please contact Hrönn, hronn.o.jorundsdottir@matis.is.

News

MARIFUNC seminar in Iceland on 19 March

On the 19th of March next. at the Hilton-Nordica Hotel, the 2nd MARIFUNC seminar will take place. The organizer of the seminar is Matís.

This is a half-day seminar where the available scientific data on seafood and health, the use and quality of seafood fats and proteins from seafood for the production of functional foods and what consumers' reactions are to seafood target foods will be reviewed. .

Seminar agenda:

What: 2 seminars of the Marifunc project on Seafood and Healthy Materials - What is the state of affairs towards consumers and companies? '
When: 19 March 2009, 8.30 - 12.45
Where: Hilton-Nordica Hotel. Suðurlandsbraut 2, Reykjavík. Meeting room E.

Registration: you need to send an email to Marifunc.registration@matis.is. The name of the participant, e-mail address and where the participant is from (city / country) must be stated. You can also register on site. Admission is free.
Registration deadline: Tuesday, March 17.

Background of the seminar:
The seminar will cover research on the effects of seafood and seafood ingredients on health. The use and quality of fats and proteins used as ingredients in supplements and target foods will also be discussed. The presentation at the seminar is based on the results of the Nordic Network for Marine Functional Food project (MARIFUNC) under the auspices of the Nordic Innovation Center. The seminar is organized by Matís ohf. (www.matis.is) and the Laboratory of Nutrition (www.rin.hi.is).

Schedule (all presentations will be in English): 8.30 Registration and coffee
9.00  Opening and presentation. Sjöfn Sigurgísladóttir, CEO of Matís ohf., Iceland.
9.10  Introducing MARIFUNC. Joop Luten, Coordinator MARIFUNC, Nofima Marine, Norway.
9.25 Seafood and health- What's the news? Alfons Ramel Laboratory of Nutrition, University of Iceland, Landspítali.
10.25 Coffee break
10.45 Challenges in the use of seafood fats in targeted foods and supplements.  Nina Skall Nielsen, DTU Aqua, Denmark
11.30 Fish protein and peptide products - processing methods, quality and processing properties. Guðjón Þorkelsson / Hörður Kristinsson, Matis ohf., Iceland.
12.15 1-2-3-4 Health. Ola Eide, Olivita, Norway.
12.35 Closing of the seminar

Here is an interesting link on the topic of the seminar.

For further information, please contact Guðjón Þorkelsson, Division Manager, gudjon.thorkelsson@matis.is.

News

Matís at Framadögur 2009

Future Days 2009 will be held on Friday 20 February in the premises of Háskólabíós.

Due to enormous changes in the Icelandic economy and economic situation, a record participation among students can be expected this year. Framadagar is therefore an ideal platform for companies to reach the future workforce of the nation by introducing themselves and their activities and thus gain a certain advantage over the competitive parties in the race for the most qualified staff.

More information can be found at www.framadagar.is and with Jón Haukur Arnarson, jon.h.arnarson@matis.is or Steinar B. Aðalbjörnsson, steinar.b.adalbjornsson@matis.is.

News

The project "Supplements - the other side" was one of the projects nominated for the President of Iceland's Innovation Award in 2009

The project was funded by the Student Innovation Fund in the summer of 2008 and was carried out by Hugrún Lísa Heimisdóttir, a student who had completed her first year in biotechnology in the field of resources at the University of Akureyri. The student's project was part of a larger project, “Bætibakteríur í lúðueldi”, which was carried out in collaboration with Matís ohf., The University of Akureyri, Fiskey hf and the University of Hólar with a grant from the Technology Development Fund (2006-2008).

The aim of the project as a whole was to look for possible beneficial bacteria in the halibut farming of Fiskey hf. and which could then be added to the environment of halibut larvae for the purpose of improving the growth and performance of the larvae.

The first few weeks are the main bottleneck in halibut farming and other marine fish, but then the larvae feed on live feed animals. The forage animals carry a large number of bacteria and the larvae at this stage of development need to rely solely on a non-specific immune response. There is therefore great interest in the use of supplementary bacteria in the aim of controlling the composition of bacterial flora in the environment and gastrointestinal tract of larvae and making it more positive for the larvae.

Treatment with a mixture of three types of potential beneficial bacteria turned out to give significantly better results to halibut larvae in the early stages of rearing, and in the student's project the focus was therefore on the other side of the beneficial bacteria, ie. the aspect of the bacterial properties that can be studied and measured in the laboratory. One of the characteristics of powerful supplemental bacteria is to inhibit the growth of unwanted bacteria and the student researched this and various other properties of the bacteria. It is also important to look for cost-effective ways to produce the bacteria in large quantities and store and transport them without losing their properties. Freeze-drying is a convenient and desirable way to store and transport bacteria, and the student examined the growth of the bacteria and their growth-inhibiting effect in both fresh and freeze-dried cultures.

The main results of the project showed that the supplementary bacteria had an inhibitory effect on the growth of dominant bacteria in some samples but little or no effect on the growth of dominant bacteria in other samples. This could indicate that in some larvae the bacterial flora is rich, which has gained a good foothold there and therefore it is difficult to influence the growth of the bacteria. Therefore, it is important to treat with supplemental bacteria early in the breeding process in order for the desired bacteria to gain a foothold there. The results also indicated that the three bacterial strains grow better in the presence of each other than alone.

It can therefore be said that the student's project has shed clear light on the properties of the additive bacteria and the most suitable methods of their production for use in the early stages of halibut farming.

The project was carried out under the guidance of Rannveig Björnsdóttir, lecturer at the University of Akureyri and other experts at Matís ohf. in Akureyri. For further information, contact Rannveig at 422-5108.

Reports

Assessment of slaughter in sheep slaughterhouses in the autumn of 2008

Published:

10/02/2009

Authors:

Valur Norðri Gunnlaugsson, Óli Þór Hilmarsson, Ásbjörn Jónsson, Guðjón Þorkelsson

Supported by:

National Association of Sheep Farmers

Contact

Valur Norðri Gunnlaugsson

Research Group Leader

valur.n.gunnlaugsson@matis.is

Assessment of slaughter in sheep slaughterhouses in the autumn of 2008

The Annual General Meeting of the National Association of Sheep Farmers decided in April 2008 that a detailed assessment will be made of the treatment of lamb at slaughter, especially during killing and cooling. An explanatory memorandum to the resolution stated: "The meeting considers it necessary to examine the possible effects of these two factors on the quality of the meat. When the animal is killed by electricity, there is a risk that the carcasses will not be able to bleed sufficiently and there is a risk of cooling hardening with too rapid cooling or freezing. Matís ohf. carried out an assessment of the above factors in the autumn of 2008 where the killing and cooling processes in 6 slaughterhouses were examined, of which one house was visited twice. Monitor the killing of 100 carcasses in each house to see procedures and take out facilities. The acidity and temperature of the carcass were measured regularly as well as the temperature in the slaughterhouses of the slaughterhouses. Carcasses taken in this study followed jogging through the normal process of action in each slaughterhouse, but before freezing, the backbone was removed and frozen. Vertebral muscles were then used in texture measurements to see different gravitational processes between slaughterhouses on the meat of the meat. The results show that the killing method affects the death stiffening process. It was much further in the carcasses of lambs in houses that use the "head-back" method than in houses that had a head clip. Cooling time is clearly too short in some houses. Thus the freezing temperature was highest as it was shortest and well above 6.0 in the house where it was only 4 hours. The viscosity of the meat was lowest in the vertebral carcasses from the slaughterhouse, where the head-back killing method was used, electrical stimulation was used and extensive and long cooling ensured that the meat was almost completely tenderized.

At annual general meeting of sheep farmers association in 2008 was concluded that a general observation ought to take place on treatment of lamb meat at slaughterhouses, particularly at electrocute step and the cooling phase. The aim was to see the influence of these factors on meat quality. Matis ohf. visited 6 slaughterhouses in autumn 2008. The results showed that the electrocution method affected the pH of carcasses. In some slaughterhouses the cooling phase was too short and therefore the pH was too high in carcasses when they were frozen. The tenderest meat came from the slaughterhouse where the meat was electrically stimulated and there was a long cooling paste.

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News

Meeting between the business community and the Knowledge Center - Matís participates

The Knowledge Center of the Westman Islands invites representatives of the business community and the public to an open working meeting on 9 February at 17.00 in Alþýðuhúsið.

The aim of the meeting is to introduce the public and representatives of the business community in the Westman Islands to the activities of the Westman Islands Knowledge Center. Discussion groups will also be set up to discuss ideas for projects and how such ideas can become a reality.

Agenda:
A. Introduction of ÞSV employees to their institution or company
1. Vestmannaeyjar Knowledge Center
2. The research service
3. Matís
4. The Business Development Association

Short break

5. Náttúrustofa
6. Surtseyjarstofa
7. The Marine Research Institute
8. Whisper
9. Iceland Innovation Center

B.      Coffee break, posters and slide show

C.      Discussion groups - Let's strengthen the community through good cooperation
Fisheries and employment
Nature and tourism
Education

Three working groups where ÞSV employees and representatives from the business community lead discussions in each group and set out ideas, goals, proposals, etc. Everyone is free to participate in discussion groups.

At the end of the meeting, a short summary will be presented from each discussion group.

Meeting adjourned.

EN